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Int J Syst Evol Microbiol 57 (2007), 675-681; DOI  10.1099/ijs.0.64767-0
© 2007 International Union of Microbiological Societies

Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water

Kwang Kyu Kim, Long Jin, Hee Chan Yang and Sung-Taik Lee

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea

Correspondence
Sung-Taik Lee
e_stlee{at}kaist.ac.kr

A total of 34 Halomonas strains were isolated from saline water in Anmyeondo, Korea. Ten of these strains, considered to belong to novel species, were subjected to a polyphasic taxonomic investigation. The strains were Gram-negative, moderately halophilic, motile and non-spore-forming rods that contained Q-9 as the predominant ubiquinone and C18 : 1{omega}7c, C16 : 0 and either summed feature 4 (C16 : 1{omega}7c/C15 : 0 iso 2-OH) or C19 : 0 cyclo {omega}8c as the major fatty acids. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the ten isolates formed four separate lineages in the genus Halomonas. Combined phenotypic data and DNA–DNA hybridization data supported the conclusion that they represent four novel species in the genus Halomonas, for which the names Halomonas gomseomensis sp. nov. (type strain M12T=KCTC 12662T=DSM 18042T), Halomonas janggokensis sp. nov. (type strain M24T=KCTC 12663T=DSM 18043T), Halomonas salaria sp. nov. (type strain M27T=KCTC 12664T=DSM 18044T) and Halomonas denitrificans sp. nov. (type strain M29T=KCTC 12665T=DSM 18045T) are proposed.


The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of H. gomseomensis M12T, H. janggokensis M24T, H. salaria M27T and H. denitrificans M29T are AM229314–AM229317, respectively.

Transmission electron micrographs of cells of strains M12T, M24T, M27T and M29T, detailed DNA–DNA hybridization results and results of API ZYM tests are available as supplementary material in IJSEM Online.




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