HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wang, Q.-f. Articles by Guo, G.-q. Search for Related Content PubMed PubMed Citation Articles by Wang, Q.-f. Articles by Guo, G.-q. Agricola Articles by Wang, Q.-f. Articles by Guo, G.-q.

Halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample

Qian-fu Wang^1,, Wei Li^1,, Hai Yang¹, Yan-li Liu¹, Hai-hua Cao¹, Marion Dornmayr-Pfaffenhuemer², Helga Stan-Lotter² and Guang-qin Guo¹

¹ Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou 730000, P. R. China
² Fachbereich Molekulare Biologie, Abt. Mikrobiologie, Billrothstrasse 11, A-5020 Salzburg, Austria

Correspondence
Guang-qin Guo
gqguo{at}lzu.edu.cn

A Gram-negative, extremely halophilic, coccoid archaeal strain, CM5^T, was isolated from a crude sea-salt sample collected near Qingdao, China. The organism grew optimally at 35–40 °C and pH 6.0 in the presence of 20 % (w/v) NaCl. Its colonies were red in colour and it could use glucose as a sole carbon source for growth. The 16S rRNA gene sequence of CM5^T was most closely related to those of Halococcus species. Its pattern of antibiotic susceptibility was similar to those of other described Halococcus species. Biochemical tests revealed no sign of H₂S production or gelatin liquefaction. The main polar lipids of strain CM5^T were phosphatidylglycerol, phosphatidylglycerol methylphosphate and sulfated diglycosyl diether. No phosphatidylglycerol sulfate was present. The DNA G+C content of strain CM5^T was 61.2 mol% and it gave DNA–DNA reassociation values of 33.7, 57.1 and 29.6 %, respectively, with Halococcus salifodinae DSM 8989^T, Halococcus dombrowskii DSM 14522^T and Halococcus morrhuae ATCC 17082^T. Based on its morphological and chemotaxonomic properties and phylogenetic analysis of 16S rRNA gene sequence data, we propose that CM5^T should be classified within a novel species, Halococcus qingdaonensis sp. nov., with strain CM5^T (=CGMCC 1.4243^T=JCM 13587^T) as the type strain.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CM5^T is AY243109.

This article has been cited by other articles:

				A. Oren, D. R. Arahal, and A. Ventosa Emended descriptions of genera of the family Halobacteriaceae Int J Syst Evol Microbiol, March 1, 2009; 59(3): 637 - 642. [Abstract] [Full Text] [PDF]

				S. Namwong, S. Tanasupawat, W. Visessanguan, T. Kudo, and T. Itoh Halococcus thailandensis sp. nov., from fish sauce in Thailand Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2199 - 2203. [Abstract] [Full Text] [PDF]