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Aeromonas bivalvium sp. nov., isolated from bivalve molluscs

Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain

Correspondence
J. Gaspar Lorén
jgloren{at}ub.edu

A polyphasic study was performed to determine the taxonomic position of two Aeromonas strains, 665N and 868E^T, isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus Aeromonas. Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E^T clustered together with a similarity of 78 % but did not cluster with any of the Aeromonas genomospecies. DNA–DNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related Aeromonas genomospecies (30–44 %). Useful tests for the phenotypic differentiation of strains 665N and 868E^T from other mesophilic Aeromonas species included those for gas from glucose, lysine decarboxylase, Voges–Proskauer reaction, acid from L-arabinose, hydrolysis of aesculin and utilization of L-lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868E^T are considered to represent a novel species of the genus Aeromonas, for which the name Aeromonas bivalvium sp. nov. is proposed. The type strain is 868E^T (=CECT 7113^T=LMG 23376^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 868E^T and 665N are DQ504429 and DQ504430, respectively.

A table giving levels of DNA–DNA hybridization between strains 868E^T and 665N and strains of phylogenetically related Aeromonas species is available as supplementary material in IJSEM Online.

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