HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Material Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Svobodová, M. Articles by Votypka, J. Search for Related Content PubMed PubMed Citation Articles by Svobodová, M. Articles by Votypka, J. Agricola Articles by Svobodová, M. Articles by Votypka, J.

Sergeia podlipaevi gen. nov., sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of biting midges (Ceratopogonidae, Diptera)

Milena Svobodová¹, Lenka Zídková¹, Ivan epika², Miroslav Oborník³, Julius Luke^3,4 and Jan Votpka^1,3

¹ Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic
² Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic
³ Biology Centre, Institute of Parasitology, Czech Academy of Sciences, eské Budjovice (Budweis), Czech Republic
⁴ Faculty of Biology, University of South Bohemia, eské Budjovice (Budweis), Czech Republic

Correspondence
Jan Votpka
vapid{at}natur.cuni.cz

Three strains of a trypanosomatid protozoan were isolated from the midguts of two naturally infected species of biting midges [Culicoides (Oecacta) festivipennis and Culicoides (Oecacta) truncorum] and characterized by light and electron microscopy and by molecular techniques. Morphological characteristics and sequences of the 18S rRNA, 5S rRNA, spliced leader RNA and glycosomal glyceraldehyde-3-phosphate dehydrogenase genes indicate that the studied flagellates represent a novel phylogenetic lineage within the Trypanosomatidae. Based on phylogenetic analyses, the novel endosymbiont-free, monoxenous trypanosomatid was classified as Sergeia podlipaevi gen. nov., sp. nov. Interestingly, it is closely related to another trypanosomatid species that parasitizes the sand fly Lutzomyia evansi, a blood-sucking dipteran from South America. The type strain of S. podlipaevi sp. nov., ICUL/CZ/2000/CER3, was obtained from Malpighian tubes. Of 2518 females of seven species of biting midges trapped in the Czech Republic, more than 1.5 % were infected by trypanosomatid parasites. An unrelated insect species, Culicoides (Monoculicoides) nubeculosus, was experimentally infected with S. podlipaevi, demonstrating that its host range extends to different subgenera of biting midges.

The GenBank/EMBL/DDBJ accession numbers for the 18S, 5S and SL RNA and partial gGAPDH gene sequences of Sergeia podlipaevi gen. nov., sp. nov. are DQ394362, DQ394363, DQ394364 and DQ394365, respectively.

A table detailing the GenBank accession numbers for the 18S, 5S and SL RNA and partial gGAPDH gene sequences analysed in this study and the composition of the final combined dataset is available as Supplementary Table S1 in IJSEM Online. Two figures, a representative RAPD gel and a phylogenetic tree inferred from the dataset of all the RAPD patterns, are also available as supplementary material.