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Sphingomonas molluscorum sp. nov., a novel marine isolate with antimicrobial activity

¹ Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
² Tokyo University of Agriculture, Department of Applied Biology and Chemistry, Faculty of Applied Bioscience, Laboratory of Food Science and Technology, Sakuragaoka 1-1-1, Setagaya-ku, Tokyo 156-8502, Japan
³ Department of Medical and Life Science, Faculty of Pharmaceutical Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan
⁴ Institute of Marine Biology, Far-Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, Russia

Correspondence
Lyudmila A. Romanenko
lro{at}piboc.dvo.ru

An aerobic, Gram-negative, yellow-pigmented, non-motile bacterium, designated strain KMM 3882^T, was isolated from a marine bivalve (Anadara broughtoni) collected from Peter the Great Bay, Sea of Japan, and was subjected to phenotypic and phylogenetic analyses. Strain KMM 3882^T was found to exert a remarkable inhibitory activity against a number of Gram-positive micro-organisms. Phylogenetic analysis based on 16S rRNA gene sequences placed strain KMM 3882^T within the genus Sphingomonas, as an independent lineage adjacent to Sphingomonas dokdonensis DS-4^T and Sphingomonas panni DSM 15761^T. Strain KMM 3882^T showed the highest 16S rRNA gene sequence similarity to Sphingomonas dokdonensis DS-4^T (97.3 %); similarities of 96.5–96.7 % were obtained with Sphingomonas pituitosa DSM 13101^T, Sphingomonas azotifigens NBRC 15497^T, Sphingomonas asaccharolytica NBRC 15499^T, Sphingomonas trueperi DSM 7225^T and Sphingomonas panni DSM 15761^T. Chemotaxonomically, strain KMM 3882^T contained sphingoglycolipid, C_{16 : 0} and C_{18 : 1} as predominant fatty acids and 2-OH C_{14 : 0} as a major 2-hydroxy fatty acid, confirming the affiliation of strain KMM 3882^T with the genus Sphingomonas. On the basis of phylogenetic analysis, DNA–DNA hybridization and physiological and biochemical characterization, strain KMM 3882^T should be classified as representing a novel species of the genus Sphingomonas, for which the name Sphingomonas molluscorum sp. nov. is proposed. The type strain is KMM 3882^T (=An 18^T=NRIC 0685^T=JCM 14122^T=CIP 109223^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KMM 3882^T is AB248285.

Tables detailing the antimicrobial properties of strain KMM 3882^T and cellular fatty acid profiles of strain KMM 3882^T and the type strains of related Sphingomonas species, and figures showing a chromatogram of the polar lipids of strain KMM 3882^T and phylogenetic trees based on 16S rRNA gene sequences constructed by using maximum-likelihood and maximum-parsimony are available as supplementary material in IJSEM Online.

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