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Int J Syst Evol Microbiol 57 (2007), 353-357; DOI  10.1099/ijs.0.64638-0
© 2007 International Union of Microbiological Societies

Nitrogen-fixing and cellulose-producing Gluconacetobacter kombuchae sp. nov., isolated from Kombucha tea

Debasree Dutta and Ratan Gachhui

Department of Life Science & Biotechnology, Jadavpur University, Kolkata 700032, West Bengal, India

Correspondence
Ratan Gachhui
ratangachhui{at}yahoo.com

A few members of the family Acetobacteraceae are cellulose-producers, while only six members fix nitrogen. Bacterial strain RG3T, isolated from Kombucha tea, displays both of these characteristics. A high bootstrap value in the 16S rRNA gene sequence-based phylogenetic analysis supported the position of this strain within the genus Gluconacetobacter, with Gluconacetobacter hansenii LMG 1527T as its nearest neighbour (99.1 % sequence similarity). It could utilize ethanol, fructose, arabinose, glycerol, sorbitol and mannitol, but not galactose or xylose, as sole sources of carbon. Single amino acids such as L-alanine, L-cysteine and L-threonine served as carbon and nitrogen sources for growth of strain RG3T. Strain RG3T produced cellulose in both nitrogen-free broth and enriched medium. The ubiquinone present was Q-10 and the DNA base composition was 55.8 mol% G+C. It exhibited low values of 5.2–27.77 % DNA–DNA relatedness to the type strains of related gluconacetobacters, which placed it within a separate taxon, for which the name Gluconacetobacter kombuchae sp. nov. is proposed, with the type strain RG3T (=LMG 23726T=MTCC 6913T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and nifH gene sequences of strain RG3T are respectively AY688433 and DQ141200.






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Copyright © 2007 by the International Union of Microbiological Societies.