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Int J Syst Evol Microbiol 57 (2007), 297-301; DOI  10.1099/ijs.0.64254-0
© 2007 International Union of Microbiological Societies

Smaragdicoccus niigatensis gen. nov., sp. nov., a novel member of the suborder Corynebacterineae

Kyoko Adachi1, Atsuko Katsuta1, Satoru Matsuda1, Xue Peng1, Norihiko Misawa1, Yoshikazu Shizuri1, Reiner M. Kroppenstedt2, Akira Yokota3 and Hiroaki Kasai1

1 Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan
2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkultren GmbH, Inhoffenstrasse 7b, D-38124 Braunschweig, Germany
3 Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan

Correspondence
Hiroaki Kasai
hiroaki.kasai{at}mbio.jp

A polyphasic taxonomic approach was applied to determine the taxonomic position of a hydrocarbon-degrading actinomycete, strain Hou_blueT, which was isolated from soil samples collected from an oil spring in Niigata, Japan. The results of 16S rRNA and gyrB gene sequence comparisons indicated that strain Hou_blueT represented a novel lineage in the suborder Corynebacterineae. Colonies were malachite green-like in colour on 1/10 trypticase soy agar and the cell morphology was coccoid in all growth phases. The cell-wall diamino acid and sugar indicated chemotype IV and variation A1{gamma}. The sugars of the peptidoglycan were glycolated. The polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and some unspecified glycolipids. The organism contained two novel cyclic forms of menaquinone, smaragdiquinone A-8(H4, {omega}-cycl) and smaragdiquinone B-8(H4, dicycl). The major fatty acids were cis-9-18 : 1 (34.46 %) and 16 : 0 (25.1 %). Small amounts of 10-methyl-branched fatty acids were also present (10-methyl-17 : 0, 0.17 %), but not tuberculostearic acid (10-methyl-18 : 0), which has been shown to be present in all nocardiae. Gas-chromatographic analysis of the mycolic acid revealed a carbon-chain length of C43–C49. The DNA G+C content was 63.7 mol%. On the basis of phenotypic and phylogenetic distinctness, the organism is proposed to represent a novel genus and species, Smaragdicoccus niigatensis gen. nov., sp. nov., with the type strain Hou_blueT (=MBIC 06267T=DSM 44881T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and gyrB gene sequences of strain Hou_blueT are AB243007 and AB243008, respectively.

A scanning electron micrograph of cells of strain Hou_blueT, a phylogenetic tree based on gyrB gene sequences and structures of the novel quinones present in strain Hou_blueT are available as supplementary figures in IJSEM Online.






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Copyright © 2007 by the International Union of Microbiological Societies.