HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yang, S.-H. Articles by Kim, S.-J. Search for Related Content PubMed PubMed Citation Articles by Yang, S.-H. Articles by Kim, S.-J. Agricola Articles by Yang, S.-H. Articles by Kim, S.-J.

Shewanella donghaensis sp. nov., a psychrophilic, piezosensitive bacterium producing high levels of polyunsaturated fatty acid, isolated from deep-sea sediments

¹ Marine Biotechnology Research Centre, Korea Ocean Research & Development Institute, PO Box 29, Ansan 425-600, Republic of Korea
² Research Program for Marine Biology and Ecology, Extremobiosphere Research Center, JAMSTEC, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan

Correspondence
Sang-Jin Kim
s-jkim{at}kordi.re.kr

A Gram-negative, motile, rod-shaped, psychrophilic bacterium, LT17^T, was isolated from deep-sea sediments (3300 m depth) of the East Sea (Sea of Japan). Optimal growth of LT17^T requires the presence of 2.5 % (w/v) NaCl, a pH of 7.0–7.5 and a temperature of 17 °C. The isolate grows optimally under a hydrostatic pressure of 10 MPa and growth is possible between 0.1 and <30 MPa. The novel strain is positive in tests for catalase, oxidase, lipase, -glucosidase and gelatinase activities and reduces nitrate to nitrate. The predominant cellular fatty acids are iso-C13 : 0, iso-C15 : 0, C16 : 0, C16 : 17 and C20 : 53. The DNA G+C content of strain LT17^T is 38.8 mol%. Phylogenetic analysis of 16S rRNA gene sequences places this bacterium in the class Gammaproteobacteria, within the genus Shewanella. The closest relatives of strain LT17^T are Shewanella japonica (97.8 % gene sequence similarity), Shewanella pacifica (97.5 %), Shewanella olleyana (96.8 %), Shewanella frigidimarina (96.5 %) and Shewanella gelidimarina (95.4 %). The DNA–DNA hybridization levels between the novel isolate and its closest known phylogenetic relatives, S. japonica and S. pacifica, are lower than 14 %. On the basis of this polyphasic evidence, strain LT17^T represents a novel species of the genus Shewanella, for which the name Shewanella donghaensis sp. nov. is proposed. The type strain is LT17^T (=KCTC 10635BP^T=JCM 12524^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LT17^T is AY326275.

A graph showing the growth rate of strain LT17^T under varying pressure conditions and an extended phylogenetic tree based on 16S rRNA gene sequences are available as supplementary figures in IJSEM Online.

This article has been cited by other articles:

				S. C. Park, K. S. Baik, M. S. Kim, D. Kim, and C. N. Seong Shewanella marina sp. nov., isolated from seawater Int J Syst Evol Microbiol, August 1, 2009; 59(8): 1888 - 1894. [Abstract] [Full Text] [PDF]

				S. Hosoya, S. Suzuki, K. Adachi, S. Matsuda, and H. Kasai Paramoritella alkaliphila gen. nov., sp. nov., a member of the family Moritellaceae isolated in the Republic of Palau Int J Syst Evol Microbiol, February 1, 2009; 59(2): 411 - 416. [Abstract] [Full Text] [PDF]

				H.-W. Chang, S. W. Roh, K.-H. Kim, Y.-D. Nam, C. O. Jeon, H.-M. Oh, and J.-W. Bae Shewanella basaltis sp. nov., a marine bacterium isolated from black sand Int J Syst Evol Microbiol, August 1, 2008; 58(8): 1907 - 1910. [Abstract] [Full Text] [PDF]

				D. Kim, K. S. Baik, M. S. Kim, B.-M. Jung, T.-S. Shin, G.-H. Chung, M. S. Rhee, and C. N. Seong Shewanella haliotis sp. nov., isolated from the gut microflora of abalone, Haliotis discus hannai Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2926 - 2931. [Abstract] [Full Text] [PDF]