HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cheng, L. Articles by Zhang, H. Search for Related Content PubMed PubMed Citation Articles by Cheng, L. Articles by Zhang, H. Agricola Articles by Cheng, L. Articles by Zhang, H.

Methermicoccus shengliensis gen. nov., sp. nov., a thermophilic, methylotrophic methanogen isolated from oil-production water, and proposal of Methermicoccaceae fam. nov.

¹ Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China
² Center for Environmental Biotechnology, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China

Correspondence
Hui Zhang
zhanghuits{at}yahoo.com.cn

A thermophilic, methylotrophic methanogen, strain ZC-1^T, was isolated from the Shengli oilfield, China. Cells of strain ZC-1^T were motile cocci, 0.7–1.0 µm in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1^T suggested the presence of a proteinaceous cell wall. Strain ZC-1^T used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0–6.5, 65 °C, 0.3–0.5 M NaCl and 0.05–0.20 M MgCl₂. The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the mcrA gene of strain ZC-1^T indicated that it is related specifically to members of the family Methanosaetaceae (90.6 and 76.6 % sequence similarity, respectively). However, strain ZC-1^T failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family Methanosaetaceae. Based on these phenotypic and phylogenic characteristics, strain ZC-1^T is proposed to represent a novel genus and species, for which the name Methermicoccus shengliensis gen. nov., sp. nov. is proposed. The type strain is ZC-1^T (=CGMCC 1.5056^T=DSM 18856^T). Methermicoccaceae fam. nov. is also proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and mcrA gene sequences of strain ZC-1^T are DQ787474 and EF026570, respectively.

This article has been cited by other articles:

				S. N. Doerfert, M. Reichlen, P. Iyer, M. Wang, and J. G. Ferry Methanolobus zinderi sp. nov., a methylotrophic methanogen isolated from a deep subsurface coal seam Int J Syst Evol Microbiol, May 1, 2009; 59(5): 1064 - 1069. [Abstract] [Full Text] [PDF]