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Leuconostoc holzapfelii sp. nov., isolated from Ethiopian coffee fermentation and assessment of sequence analysis of housekeeping genes for delineation of Leuconostoc species

¹ Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium
² Federal Research Centre for Nutrition and Food, Institute of Hygiene and Toxicology, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany
³ BCCM/LMG Bacteria Collection, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium
⁴ Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Department of Applied Biological Sciences and Engineering, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium

Correspondence
Katrien De Bruyne
Katrien.DeBruyne{at}UGent.be

A Gram-positive, ovoid lactic acid bacterium, strain LMG 23990^T, was isolated from Ethiopian coffee fermentation. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the genus Leuconostoc, with Leuconostoc citreum and Leuconostoc lactis as the closest neighbours (99.6 and 99.0 % 16S rRNA gene sequence similarity, respectively). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism, whole-cell protein electrophoresis, DNA–DNA hybridizations, comparative sequence analysis of pheS, rpoA, atpA, and physiological and biochemical tests allowed us to differentiate strain LMG 23990^T from all established Leuconostoc species. Strain LMG 23990^T (=CCUG 54536^T) therefore represents a novel species, for which the name Leuconostoc holzapfelii sp. nov. is proposed.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of LMG 23990^T is AM600682. The GenBank/EMBL/DDBJ accession numbers for the pheS, rpoA and atpA gene sequences reported in this paper are AM711136–AM711355, as indicated in Supplementary Figs S1–S6.

Supplementary figures are available with the online version of this paper.

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