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Bacillus plakortidis sp. nov. and Bacillus murimartini sp. nov., novel alkalitolerant members of rRNA group 6

¹ Hamburg University of Technology, Technical Microbiology, Kasernenstrasse 12, D-21073 Hamburg, Germany
² Novozymes A/S, Bacterial Screening, Krogshøjvej 36, DK-2880 Bagsværd, Denmark
³ Berlin University of Technology, Fac. III, Microbial Ecology Group, Franklinstrasse 29, Sekr. FR 1-2, D-10587 Berlin, Germany
⁴ University of Hamburg, Institute of Biogeochemistry and Marine Chemistry, Bundesstrasse 55, D-20146 Hamburg, Germany

Correspondence
Martin S. Borchert
mtbo{at}novozymes.com

The Gram-positive, alkali- and salt-tolerant marine bacterium strain P203^T is described together with its closest phylogenetic neighbour, terrestrial isolate LMG 21005^T. Strain P203^T was isolated from material from the sponge Plakortis simplex that was obtained from the Sula-Ridge, Norwegian Sea. Strain LMG 21005^T was an undescribed strain that was isolated from a church wall mural in Germany. Strains P203^T and LMG 21005^T were identified as novel alkalitolerant members of the Bacillus rRNA group 6 with a 16S rRNA gene sequence similarity of 99.5 %. The closest described neighbour, Bacillus gibsonii DSM 8722^T, showed 99.0 % gene sequence similarity with P203^T and 98.8 % similarity with strain LMG 21005^T. Despite the high 16S rRNA gene sequence similarity, DNA–DNA cross-hybridization revealed only 25.8–34.1 % similarity amongst the three strains. The DNA G+C contents were 41.1 mol% for strain P203^T and 39.6 mol% for strain LMG 21005^T. Both strains grew well between pH 7 and pH 11. Strain P203^T showed growth at moderate temperatures (from 4 to 30 °C) and in the presence of up to 12 % (w/v) NaCl at pH 9.7, whereas strain LMG 21005^T was not salt tolerant (up to 4 % NaCl) and no growth was observed at 4 °C. The major fatty acids of strains P203^T, LMG 21005^T and the type strain of B. gibsonii were the saturated terminally methyl-branched compounds iso-C_{15 : 0} (19.8, 15.6 and 28.0 %, respectively) and anteiso-C_{15 : 0} (57.1, 48.6 and 45.2 %, respectively). Physiological and biochemical tests allowed genotypic and phenotypic differentiation of strains P203^T and LMG 21005^T from the six related Bacillus species with validly published names and supported the proposal of two novel species, Bacillus plakortidis [type strain P203^T (=DSM 19153^T=NCIMB 14288^T)] and Bacillus murimartini [type strain LMG 21005^T (=NCIMB 14102^T)].

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains Bacillus plakortidis sp. nov. DSM 19153^T and Bacillus murimartini sp. nov. LMG 21005^T are AJ880003 and AJ316316, respectively.

A table detailing the fatty acid content of strains DSM 19153^T and LMG 21005^T and electron micrographs showing the morphology of cells of the two novel strains are available with the online version of this paper.