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Int J Syst Evol Microbiol 57 (2007), 2865-2869; DOI  10.1099/ijs.0.65067-0
© 2007 International Union of Microbiological Societies

Desulfotignum toluenicum sp. nov., a novel toluene-degrading, sulphate-reducing bacterium isolated from an oil-reservoir model column

Hege Ommedal1,2 and Terje Torsvik1

1 University of Bergen, Centre for Integrated Petroleum Research (CIPR), Allégaten 41, N-5007 Bergen, Norway
2 University of Bergen, Department of Biology, Jahnebakken 5, N-5020 Bergen, Norway

Correspondence
Terje Torsvik
terje.torsvik{at}bio.uib.no

A Gram-negative, sulphate-reducing bacterium (strain H3T) was isolated from an oil-reservoir model column. The new isolate was able to oxidize toluene coupled to hydrogen sulphide production. For growth, the optimum salt concentration was 1.5 % (w/v), the optimum pH was 7.2 and the optimum temperature was 34 °C. The cells were straight to slightly curved rods, 0.6–1.0 µm in diameter and 1.4–2.5 µm in length. The predominant fatty acids were C16 : 0, C16 : 1{omega}7c and C17 : 0 cyclo, and the cells also contained dimethylacetals. Cloning and sequencing of a 1505 bp long fragment of the 16S rRNA gene showed that strain H3T is a member of the Deltaproteobacteria and is related closely to Desulfotignum balticum DSM 7044T. The G+C content of the DNA was 52.0 mol% and the DNA–DNA similarity to D. balticum DSM 7044T was 56.1 %. Based on differences in DNA sequence and the unique property of toluene degradation, it is proposed that strain H3T should be designated a member of a novel species within the genus Desulfotignum, for which the name Desulfotignum toluenicum sp. nov. is proposed. The type strain is H3T (=DSM 18732T=ATCC BAA-1460T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences determined in this study are EF207157–EF207159 for strain H3T clone sequences 1, 4 and 7, respectively.

A supplementary table showing the varying bases in the analysed Desulfotignum sp. 16S rRNA gene sequences, and supplementary figures showing a transmission electron micrograph of unstained cells of Desulfotignum toluenicum H3T and rep-PCR profiles for the analysed strains of Desulfotignum, are available with the online version of this paper.




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