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Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis

¹ Division of Environmental Science and Ecological Engineering, Korea University, Seoul, South Korea
² Biological Resource Center, KRIBB, Daejeon, South Korea
³ Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, South Korea

Correspondence
Woojun Park
wpark{at}korea.ac.kr

A novel bacterial strain, designated PMB02^T, was isolated from a leaf of the tree Platanus orientalis. Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2–1.5 mm in diameter after 3 days growth. The cells of strain PMB02^T were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20–30 °C, pH 6–8 and in media containing less than 1 % NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02^T was related to members of the genus Methylobacterium. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium aquaticum and Methylobacterium variabile, with which it showed sequence similarities of 97.7 and 97.4 %, respectively. The values for DNA–DNA hybridization between strain PMB02^T and M. aquaticum CCM 7218^T and M. variabile GR3^T were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA–DNA relatedness data, strain PMB02^T is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium platani sp. nov. is proposed. The type strain is PMB02^T (=KCTC 12901^T=JCM 14648^T).

Photomicrographs of cells of strain PMB02^T, DNA–DNA hybridization data and fatty acid profiles for strain PMB02^T and related strains are available as supplementary material with the online version of this paper.

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