HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Free via Open Access: OA OA Free Full Text Full Text (PDF) Supplementary Figures Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Morita, H. Articles by Masaoka, T. Search for Related Content PubMed PubMed Citation Articles by Morita, H. Articles by Masaoka, T. Agricola Articles by Morita, H. Articles by Masaoka, T.

Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds

¹ School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan
² Microbial Genome Research Group, Japan Agency of Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan
³ Nodai Culture Collection Center, Department of Brewing, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan
⁴ Northern Farm, 275 Hayakita-genbu, Abira-cho, Yufutsu-gun, Hokkaido 059-1432, Japan
⁵ Clossfield-Bio Inc., 1-1-20, Higashi Nihonbashi, Chuo, Tokyo 103-0004, Japan

Correspondence
Hidetoshi Morita
morita{at}azabu-u.ac.jp

Two strains, KBL13^T and GBL13, were isolated as one of intestinal lactobacilli from the faecal specimens from different thoroughbreds of the same farm where they were born in Hokkaido, Japan. They were Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods. KBL13^T and GBL13 homofermentatively metabolize glucose, and produce lactate as the sole final product from glucose. The 16S rRNA gene sequence, DNA–DNA hybridization, DNA G+C content and biochemical characterization indicated that these two strains, KBL13^T and GBL13, belong to the same species. In the representative strain, KBL13^T, the DNA G+C content was 34.3 mol%. Lactobacillus salivarius JCM 1231^T (=ATCC 11741^T; AF089108) is the type strain most closely related to the strain KBL13^T as shown in the phylogenetic tree, and the 16S rRNA gene sequence identity showed 96.0 % (1425/1484 bp). Comparative 16S rRNA gene sequence analysis of this strain indicated that the two isolated strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives, L. salivarius, Lactobacillus aviarius, Lactobacillus saerimneri and Lactobacillus acidipiscis. DNA–DNA reassociation experiments with L. salivarius and L. aviarius confirmed that KBL13^T represents a novel species, for which the name Lactobacillus hayakitensis sp. nov. is proposed. The type strain is KBL13^T (=JCM 14209^T=DSM 18933^T).

Trees showing the phylogenetic relationship, based on the 16S rRNA gene sequence, of the isolate in comparison to species of the Lactobacillus salivarius phylogenetic group constructed by the maximum-parsimony and maximum-likelihood methods are available with the online version of this paper.

This article has been cited by other articles:

				A. Endo, S. Roos, E. Satoh, H. Morita, and S. Okada Lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses Int J Syst Evol Microbiol, April 1, 2008; 58(4): 914 - 918. [Abstract] [Full Text] [PDF]