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Int J Syst Evol Microbiol 57 (2007), 2805-2809; DOI  10.1099/ijs.0.65128-0
© 2007 International Union of Microbiological Societies

Azospirillum zeae sp. nov., a diazotrophic bacterium isolated from rhizosphere soil of Zea mays

Samina Mehnaz1,2, Brian Weselowski2 and George Lazarovits2

1 School of Biological Sciences, Quaid-e-Azam Campus, University of the Punjab, Lahore 54590, Pakistan
2 Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON N5V 4T3, Canada

Correspondence
George Lazarovits
lazarovitsg{at}agr.gc.ca

Two free-living nitrogen-fixing bacterial strains, N6 and N7T, were isolated from corn rhizosphere. A polyphasic taxonomic approach, including morphological characterization, Biolog analysis, DNA–DNA hybridization, and 16S rRNA, cpn60 and nifH gene sequence analysis, was taken to analyse the two strains. 16S rRNA gene sequence analysis indicated that strains N6 and N7T both belonged to the genus Azospirillum and were closely related to Azospirillum oryzae (98.7 and 98.8 % similarity, respectively) and Azospirillum lipoferum (97.5 and 97.6 % similarity, respectively). DNA–DNA hybridization of strains N6 and N7T showed reassociation values of 48 and 37 %, respectively, with A. oryzae and 43 % with A. lipoferum. Sequences of the nifH and cpn60 genes of both strains showed 99 and ~95 % similarity, respectively, with those of A. oryzae. Chemotaxonomic characteristics (Q-10 as quinone system, 18 : 1{omega}7c as major fatty acid) and G+C content of the DNA (67.6 mol%) were also similar to those of members of the genus Azospirillum. Gene sequences and Biolog and fatty acid analysis showed that strains N6 and N7T differed from the closely related species A. lipoferum and A. oryzae. On the basis of these results, it is proposed that these nitrogen-fixing strains represent a novel species. The name Azospirillum zeae sp. nov. is suggested, with N7T (=NCCB 100147T=LMG 23989T) as the type strain.

Abbreviations: IAA, indole acetic acid

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, cpn60 and nifH gene sequences of strain N6 are DQ682469, DQ682474 and DQ682471, respectively; the corresponding accession numbers for strain N7T are DQ682470, DQ682473 and DQ682472.






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