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Int J Syst Evol Microbiol 57 (2007), 2799-2804; DOI  10.1099/ijs.0.65192-0
© 2007 International Union of Microbiological Societies

Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius

Martha E. Trujillo1, Reiner M. Kroppenstedt2, Carmen Fernández-Molinero1, Peter Schumann2 and Eustoquio Martínez-Molina1

1 Departamento de Microbiología y Genética, Edificio Departamental, Lab. 209, Campus Miguel de Unamuno, Universidad de Salamanca, Salamanca, Spain
2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany

Correspondence
Martha E. Trujillo
mett{at}usal.es

A study was conducted to determine the taxonomic status of six actinomycete strains isolated from root nodules of Lupinus angustifolius. The strains were filamentous, Gram-positive and produced single spores at the tip of the hyphae. Phylogenetic, chemotaxonomic and morphological analyses demonstrated that all six strains belonged to the genus Micromonospora. According to the 16S rRNA gene sequence data, the strains were divided into two clusters that are moderately related to Micromonospora mirobrigensis, Micromonospora matsumotoense and Micromonospora purpureochromogenes. Fatty acid patterns also supported the division of the strains, and significant differences between the two groups were found in the amounts of iso-15 : 0, iso-16 : 0, iso-16 : 1 and iso-17 : 0. Furthermore, the two groups showed physiological differences which included utilization of arabinose, trehalose, alanine and sucrose and xylan hydrolysis. Finally, DNA–DNA hybridization and ribotyping studies confirmed that each group represents a novel species. Based on the genotypic and phenotypic data, the novel species Micromonospora lupini sp. nov. (type strain Lupac 14NT =DSM 44874T =LMG 24055T) and Micromonospora saelicesensis sp. nov. (type strain Lupac 09T =DSM 44871T =LMG 24056T) are proposed.

Abbreviations: DAP, diaminopimelic acid

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA sequences of isolates Lupac 06, Lupac 07, Lupac 08, Lupac 09T, Lupac 13 and Lupac 14NT are respectively AJ783990–AJ783993, AJ783995 and AJ783996.

Scanning electron micrographs of spores of strains Lupac 09T and Lupac 14NT, an extended phylogenetic tree, cultural characteristics of the novel strains and detailed fatty acid profiles are available as supplementary material with the online version of this paper.






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Copyright © 2007 by the International Union of Microbiological Societies.