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Leucobacter chromiireducens subsp. solipictus subsp. nov., a pigmented bacterium isolated from the nematode Caenorhabditis elegans, and emended description of L. chromiireducens

¹ Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA
² Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5120, USA

Correspondence
Man-Wah Tan
mwtan{at}stanford.edu

A yellow-pigmented, Gram-positive, aerobic, non-motile, non-spore-forming, irregular rod-shaped bacterium (strain TAN 31504^T) was isolated from the bacteriophagous nematode Caenorhabditis elegans. Based on 16S rRNA gene sequence similarity, DNA G+C content of 69.5 mol%, 2,4-diaminobutyric acid in the cell-wall peptidoglycan, major menaquinone MK-11, abundance of anteiso- and iso-fatty acids, polar lipids diphosphatidylglycerol and phosphatidylglycerol and a number of shared biochemical characteristics, strain TAN 31504^T was placed in the genus Leucobacter. DNA–DNA hybridization comparisons demonstrated a 91 % DNA–DNA relatedness between strain TAN 31504^T and Leucobacter chromiireducens LMG 22506^T indicating that these two strains belong to the same species, when the recommended threshold value of 70 % DNA–DNA relatedness for the definition of a bacterial species by the ad hoc committee on reconciliation of approaches to bacterial systematics is considered. Based on distinct differences in morphology, physiology, chemotaxonomic markers and various biochemical characteristics, it is proposed to split the species L. chromiireducens into two novel subspecies, Leucobacter chromiireducens subsp. chromiireducens subsp. nov. (type strain L-1^T=CIP 108389^T=LMG 22506^T) and Leucobacter chromiireducens subsp. solipictus subsp. nov. (type strain TAN 31504^T=DSM 18340^T=ATCC BAA-1336^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain TAN 31504^T is DQ845457.

Transmission electron micrographs of cells of strain TAN 31504^T and a neighbour-joining tree based on 16S rRNA gene sequences showing the relationship of strain TAN 31504^T to members of the genus Leucobacter and other reference organisms are available as supplementary figures with the online version of this paper.

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				R. E. Muir and M.-W. Tan Virulence of Leucobacter chromiireducens subsp. solipictus to Caenorhabditis elegans: Characterization of a Novel Host-Pathogen Interaction Appl. Envir. Microbiol., July 1, 2008; 74(13): 4185 - 4198. [Abstract] [Full Text] [PDF]