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Schlesneria paludicola gen. nov., sp. nov., the first acidophilic member of the order Planctomycetales, from Sphagnum-dominated boreal wetlands

¹ S. N. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letya Octyabrya 7/2, Moscow 117312, Russia
² M. V. Lomonosov Moscow State University, Faculty of Biology Science, GSP-2, Leninskie Gory, Moscow 119992, Russia
³ Netherlands Institute of Ecology, 3631 AC Nieuwersluis, The Netherlands
⁴ Royal Netherlands Institute for Sea Research, Department of Marine Biogeochemistry and Toxicology, PO Box 59, 1790 AB Den Burg, The Netherlands

Correspondence
Svetlana N. Dedysh
dedysh{at}mail.ru

Three strains of budding, ellipsoid-shaped and rosette-forming bacteria were isolated from acidic Sphagnum-dominated boreal wetlands of northern Russia and were designated strains MPL7^T, MOB77 and SB2. The presence of crateriform pits and numerous fibrillar appendages on the cell surface and an unusual spur-like projection on one pole of the cell indicated a planctomycete morphotype. These isolates are moderately acidophilic, mesophilic organisms capable of growth at pH values between 4.2 and 7.5 (with an optimum at pH 5.0–6.2) and at temperatures between 4 and 32 °C (optimum 15–26 °C). The major fatty acids are C_{16 : 0} and C_{16 : 1}7c; the major quinone is MK-6. The G+C content of the DNA is 54.4–56.5 mol%. Strains MPL7^T, MOB77 and SB2 possess nearly identical 16S rRNA gene sequences and belong to the planctomycete lineage defined by the genus Planctomyces, being most closely related to Planctomyces limnophilus DSM 3776^T (86.9–87.1 % sequence similarity). However, strain MPL7^T showed only 28 % DNA–DNA hybridization with P. limnophilus DSM 3776^T. Compared with currently described members of the genus Planctomyces, the isolates from northern wetlands do not form long and distinctive stalks, have greater tolerance of acidic conditions and low temperatures, are more sensitive to NaCl, lack pigmentation and degrade a wider range of biopolymers. The data therefore suggest that strains MPL7^T, MOB77 and SB2 represent a novel genus and species, for which the name Schlesneria paludicola gen. nov., sp. nov., is proposed. Strain MPL7^T (=ATCC BAA-1393^T =VKM B-2452^T) is the type strain of Schlesneria paludicola.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MPL7^T is AM162407.

Additional electron micrographs and temperature and pH growth profiles are available as supplementary material with the online version of this paper.

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				I. S. Kulichevskaya, A. O. Ivanova, O. I. Baulina, P. L. E. Bodelier, J. S. S. Damste, and S. N. Dedysh Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands Int J Syst Evol Microbiol, May 1, 2008; 58(5): 1186 - 1193. [Abstract] [Full Text] [PDF]