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Shimazuella kribbensis gen. nov., sp. nov., a mesophilic representative of the family Thermoactinomycetaceae

Dong-Jin Park^1,, Syed G. Dastager^1,, Jae-Chan Lee¹, Soo-Hwan Yeo², Jung-Hoon Yoon¹ and Chang-Jin Kim¹

¹ Korea Research Institutes of Biosciences and Biotechnology, 52 Eoeun-dong, Yuseong gu, Daejeon 305-333, Republic of Korea
² Rural Resources Development Institute, National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon 411-853, Republic of Korea

Correspondence
Chang-Jin Kim
changjin{at}kribb.re.kr

A mesophilic strain, designated A 9500^T, was isolated from a soil sample collected from Sobaek Mountain, South Korea, and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew well on different ISP media and no diffusible pigments were produced. The optimum temperature for growth was 32 °C. The aerial mycelium was well developed, but not fragmented. The strain was Gram-positive, non-motile and formed endospores on vegetative and aerial hyphae with a spiny surface. Cell walls of strain A 9500^T contained meso-diaminopimelic acid as the diagnostic amino acid, but no characteristic sugars or other amino acids were found (chemotype-III). The major menaquinone was MK-9(H₄) and the minor menaquinone was MK-10(H₄) and they were detected at a ratio of 7 : 3. Phosphatidylethanolamine was the diagnostic phospholipid. The G+C content of the genomic DNA was 39.4 mol%. The major fatty acids were anteiso-C_{15 : 0} (43.34 %), iso-C_{16 : 0} (14.23 %) and C_{16 : 0} (7.90 %), a composition that differed from members of related genera of the family Thermoactinomycetaceae. The novel strain formed a distinct clade in a phylogenetic tree based on 16S rRNA gene sequences. On the basis of a range of phenotypic and genotypic data and on 16S rRNA gene sequence similarities (88.35–90.38 %), it is suggested that strain A 9500^T represents a novel species in a new genus with the name Shimazuella kribbensis gen. nov., sp. nov. The type strain of the type species is A 9500^T (=KCTC 9933^T=DSM 45090^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A 9500^T is AB049939.

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