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Actinocatenispora sera sp. nov., isolated by long-term culturing

Atsuko Matsumoto¹, Yko Takahashi², Megumi Fukumoto² and Satoshi mura^1,2

¹ The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan
² Kitasato Institute for Life Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan

Correspondence
Yko Takahashi
ytakaha{at}lisci.kitasato-u.ac.jp

Two novel actinomycete strains, KV-744^T and KV-856, were isolated by long-term cultivation. Aerial long-chain spores were produced directly from vegetative mycelia and possessed no motility. Vegetative mycelia developed very well and exhibited fragmentation. The cell-wall peptidoglycan contained meso-diaminopimelic acid, glycine, alanine and glutamic acid, and whole-cell hydrolysates contained arabinose, galactose and xylose. The acyl type of the peptidoglycan was glycolyl. The predominant menaquinone was MK-9(H₄) and mycolic acids were not detected. The diagnostic phospholipid was phosphatidylethanolamine. The predominant cellular fatty acids were 14-methylhexadecanoic (ai-C_{17 : 0}), 14-methylpentadecanoic (i-C_{16 : 0}), 15-methylhexadecanoic (i-C_{17 : 0}) and 13-methyltetradecanoic (i-C_{15 : 0}) acids. The G+C content of the DNA was 72–73 mol%. The phenotypic and chemical properties indicated that the two isolates belong to the family Micromonosporaceae and the 16S rRNA gene sequence analysis suggested that the closest relationship was with Actinocatenispora thailandica. The DNA–DNA hybridization values between strain KV-744^T or KV-856 and A. thailandica TT2-10^T were 42–53 %. Based on the data above, strains KV-744^T and KV-856 should be classified as representing a novel species of the genus Actinocatenispora, for which the name Actinocatenispora sera sp. nov. is proposed. The type strain is KV-744^T (=NRRL B-24477^T=NBRC 101916^T).

Scanning electron micrographs showing aerial and vegetative mycelia of strain KV-744^T are available as a supplementary figure with the online version of this paper.