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Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp. nov., isolated from industrial and haemodialysis water

¹ Área Microbiologia, Departament de Biologia, Universitat de les Illes Balears, and Institut Mediterrani d'Estudis Avançats (CSIC-UIB), 07122 Palma de Mallorca, Illes Balears, Spain
² Abt. Molekularphysiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, 37077 Göttingen, Germany
³ CCUG, Culture Collection University of Göteborg, Department of Clinical Bacteriology, 41346 Göteborg, Sweden

Correspondence
Jorge Lalucat
jlalucat{at}uib.es

Three Gram-negative, rod-shaped, non-spore-forming bacteria (strains CCUG 52769^T, CCUG 52770 and CCUG 52771) isolated from haemodialysis water were characterized taxonomically, together with five strains isolated from industrial waters (CCUG 52428, CCUG 52507, CCUG 52575^T, CCUG 52590 and CCUG 52631). Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the class Betaproteobacteria and were related to the genus Pelomonas, with 16S rRNA gene sequence similarities higher than 99 % with the only species of the genus, Pelomonas saccharophila and to Pseudomonas sp. DSM 2583. The type strains of Mitsuaria chitosanitabida and Roseateles depolymerans were their closest neighbours (97.9 and 97.3 % 16S rRNA gene sequence similarity, respectively). Phylogenetic analysis was also performed for the internally transcribed spacer region and for three genes [hoxG (hydrogenase), cbbL/cbbM (Rubisco) and nifH (nitrogenase)] relevant for the metabolism of the genus Pelomonas. DNA–DNA hybridization, major fatty acid composition and phenotypical analyses were carried out, which included the type strain of Pelomonas saccharophila obtained from different culture collections (ATCC 15946^T, CCUG 32988^T, DSM 654^T, IAM 14368^T and LMG 2256^T), as well as M. chitosanitabida IAM 14711^T and R. depolymerans CCUG 52219^T. Results of DNA–DNA hybridization, physiological and biochemical tests supported the conclusion that strains CCUG 52769, CCUG 52770 and CCUG 52771 represent a homogeneous phylogenetic and genomic group, including strain DSM 2583, clearly differentiated from the industrial water isolates and from the Pelomonas saccharophila type strain. On the basis of phenotypic and genotypic characteristics, these strains belong to two novel species within the genus Pelomonas, for which the names Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are proposed. The type strains of Pelomonas puraquae sp. nov. and Pelomonas aquatica sp. nov. are CCUG 52769^T (=CECT 7234^T) and CCUG 52575^T (=CECT 7233^T), respectively.

The GenBank/EMBL/DDBJ accession numbers for the novel strains are: CCUG 52575^T (AM51435 and AM501454, for 16S rRNA gene and ITS1, respectively), and for strain CCUG 52769^T [AM501439, AM501458, AM501467 and AM501476, for 16S rRNA gene, ITS1, nifH and red-like form I Rubisco genes (cbbL), respectively].

Supplementary data are available with the online version of this paper.

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