HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figure and Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lu, S. Articles by Jeon, C. O. Search for Related Content PubMed PubMed Citation Articles by Lu, S. Articles by Jeon, C. O. Agricola Articles by Lu, S. Articles by Jeon, C. O.

Runella defluvii sp. nov., isolated from a domestic wastewater treatment plant

Shipeng Lu^1,, Jung Ro Lee^1,, Seung Hyun Ryu¹, Bok Sil Chung¹, Woo-Seok Choe² and Che Ok Jeon¹

¹ Division of Applied Life Science, EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea
² Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea

Correspondence
Che Ok Jeon
cojeon{at}gnu.ac.kr

A long, Gram-negative, rod-shaped bacterium, designated strain EMB13^T, was isolated from a wastewater treatment plant in Korea. The isolate was strictly aerobic and non-motile. The strain grew optimally at 30–35 °C and pH 7.5–8.0, and the predominant fatty acids were iso-C_{15 : 0}, summed feature 3 (C_{16 : 1}7c and/or iso-C_{15 : 0} 2-OH), C_{16 : 1}5c and iso-C_{17 : 0} 3-OH. The strain contained a large amount of phosphatidylethanolamine and small amounts of phosphatidylcholine and an unknown phospholipid as the polar lipids. The G+C content of the genomic DNA was 40.1 mol% and the major isoprenoid quinone was menaquinone-7. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB13^T belonged to the genus Runella and was most closely related to Runella limosa EMB111^T, with a 16S rRNA gene sequence similarity of 97.1 %. DNA–DNA relatedness between strain EMB13^T and R. limosa EMB111^T was approximately 25 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB13^T represents a novel species within the genus Runella, for which the name Runella defluvii sp. nov. is proposed. The type strain is EMB13^T (=KCTC 12614^T =DSM 17976^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain EMB13^T is DQ372980.

A transmission electron micrograph of cells of strain EMB13^T and API 50CH utilization results for strain EMB13^T are available as supplementary material with the online version of this paper.