HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figures Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vaz-Moreira, I. Articles by Manaia, C. M. Search for Related Content PubMed PubMed Citation Articles by Vaz-Moreira, I. Articles by Manaia, C. M. Agricola Articles by Vaz-Moreira, I. Articles by Manaia, C. M.

Paenibacillus humicus sp. nov., isolated from poultry litter compost

¹ Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4200-072 Porto, Portugal
² Departamento de Zoologia, Universidade de Coimbra, 3004-517 Coimbra, Portugal
³ DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7b, D-38124 Braunschweig, Germany
⁴ LEPAE – Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, 4200-465 Porto, Portugal

Correspondence
Célia M. Manaia
cmmanaia{at}esb.ucp.pt

Two bacterial strains, PC-142 and PC-147^T, isolated from poultry litter compost, were characterized with respect to their phenetic and phylogenetic characteristics. The isolates were endospore-forming rods that were reddish in colour after Gram staining. They were catalase- and oxidase-positive, were able to degrade starch and gelatin and grew at 15–40 °C and pH 5.5–10.0. The predominant fatty acids were anteiso-C_{15 : 0}, iso-C_{15 : 0} and iso-C_{16 : 0}, the major respiratory quinone was menaquinone MK-7, the cell-wall peptidoglycan was of the A1 type and the G+C content of the DNA was 58 mol%. The 16S rRNA gene sequence analysis and phenetic characterization indicated that these organisms belong to the genus Paenibacillus, with Paenibacillus pasadenensis SAFN-007^T as the closest phylogenetic neighbour (97.5 %). Strains PC-142, PC-147^T and P. pasadenensis SAFN-007^T represent a novel lineage within the genus Paenibacillus, characterized by a high DNA G+C content (58–63 mol%). The low levels of 16S rRNA gene sequence similarity with respect to other taxa with validly published names and the identification of distinctive phenetic features in the two isolates indicate that strains PC-142 and PC-147^T represent a novel species of the genus Paenibacillus, for which the name Paenibacillus humicus sp. nov. is proposed. The type strain is PC-147^T (=DSM 18784^T =NBRC 102415^T =LMG 23886^T).

RAPD patterns and an extended phylogenetic tree for strains PC-142 and PC-147^T and related taxa are available as supplementary figures with the online version of this paper.

This article has been cited by other articles:

				I. Vaz-Moreira, M. F. Nobre, A. C. S. Ferreira, P. Schumann, O. C. Nunes, and C. M. Manaia Humibacter albus gen. nov., sp. nov., isolated from sewage sludge compost Int J Syst Evol Microbiol, April 1, 2008; 58(4): 1014 - 1018. [Abstract] [Full Text] [PDF]