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Micrococcus flavus sp. nov., isolated from activated sludge in a bioreactor

¹ State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences, Zhong-Guan-Cun, Haidian, Beijing 100080, China
² Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Correspondence
Shuang-Jiang Liu
liusj{at}sun.im.ac.cn

Bacterial strain LW4^T was isolated from activated sludge of a wastewater-treatment bioreactor. Cells of strain LW4^T were Gram-positive cocci, with a diameter of 0.7–1.0 µm. Colonies produced on LB agar plates were yellow, smooth, circular and 0.5–1.5 mm in diameter. Strain LW4^T was aerobic and grew over the temperature range 26–34 °C and pH range 5–9, with optimal growth at 30.5–31.5 °C and pH 6.0–6.2. The cell-wall peptidoglycan of strain LW4^T contained amino acid residues of lysine, glutamic acid, alanine, glycine and aspartic acid. The most abundant cellular fatty acids of strain LW4^T were anteiso-C_{15 : 0} (32.15 %) and iso-C_{15 : 0} (31.65 %). Major respiratory quinones were MK-8(H₂) (57.3 %) and MK-7(H₂) (32.9 %). The DNA G+C content was 71.4 mol% (T_m). 16S rRNA gene sequence analysis indicated that strain LW4^T was phylogenetically related to members of the genus Micrococcus, with similarities ranging from 96.5 to 97.3 %. Levels of DNA–DNA relatedness of strain LW4^T to Micrococcus luteus DSM 20030^T, Micrococcus lylae DSM 20315^T and Micrococcus antarcticus AS 1.2372^T were 55, 48 and 36 %, respectively. Based on these results, it is concluded that strain LW4^T represents a novel species of the genus Micrococcus, for which the name Micrococcus flavus sp. nov. is proposed. The type strain is strain LW4^T (=CGMCC 1.5361^T=JCM 14000^T).

A scanning electron micrograph of cells of strain LW4^T and a table detailing its cellular fatty acid composition are available as supplementary material in IJSEM Online.