| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, P. R. China
2 State Key Laboratory of Marine Geology, Tongji University, Shanghai, P. R. China
3 Center for Marine Biotechnology and Biomedicine, Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093-0202, USA
Correspondence
Fengping Wang
fengpingw{at}yahoo.com
Two Shewanella-like bacterial strains, WP2T and WP3T, which were isolated from west Pacific deep-sea sediment, were studied to determine their taxonomic position. Cells of the two bacteria were facultatively anaerobic, Gram-negative rods and motile by means of a single polar flagellum. Strain WP2T was psychrophilic, growing optimally at about 10–15 °C, whereas strain WP3T was psychrotolerant, growing optimally at 15–20 °C. The two strains grew in the pressure range 0.1–50 MPa, with optimal growth at 20 MPa. Strain WP3T was able to use nitrate, fumarate, trimethylamine N-oxide (TMAO), DMSO and insoluble Fe(III) as terminal electron acceptors during anaerobic growth, whereas strain WP2T was able to use only nitrate, TMAO and DMSO. The 16S rRNA gene sequences of strains WP2T and WP3T were 97 % identical, and showed highest similarity (97 %) to those of Shewanella fidelis KMM 3589 and Shewanella benthica ATCC 43992T, respectively. The gyrB gene sequences of strains WP2Tand WP3 T were also determined, and showed highest similarity to those of Shewanella violacea JCM 10179T (90 %) and Shewanella sairae SM2-1T (87 %), respectively. Contrary to the 16S rRNA gene sequence results, the phylogeny based on gyrB gene sequence analysis placed strain WP2T, S. violacea and S. benthica in one group, while strain WP3T grouped with S. fidelis and S. sairae. DNA–DNA hybridization experiments supported the placement of strain WP2T with S. violacea and S. benthica. Phylogenetic evidence, together with DNA–DNA relatedness and phenotypic characteristics, indicated that the two new strains represented two novel deep-sea Shewanella species. The names Shewanella psychrophila sp. nov. (type strain WP2T=JCM 13876T=CGMCC 1.6159T) and Shewanella piezotolerans (type strain WP3T=JCM 13877T=CGMCC 1.6160T) are proposed.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and gyrB gene sequences of Shewanella psychrophila WP2T are AJ551089 and AM229307, and those of Shewanella piezotolerans WP3T are AJ551090 and AM229308, respectively.
This article has been cited by other articles:
|
|
 |
|
  F. Wang, Y. Gai, M. Chen, and X. Xiao Arthrobacter psychrochitiniphilus sp. nov., a psychrotrophic bacterium isolated from Antarctica Int J Syst Evol Microbiol, November 1, 2009; 59(11): 2759 - 2762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Park, K. S. Baik, M. S. Kim, D. Kim, and C. N. Seong Shewanella marina sp. nov., isolated from seawater Int J Syst Evol Microbiol, August 1, 2009; 59(8): 1888 - 1894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Wang, X. Xiao, H.-Y. Ou, Y. Gai, and F. Wang Role and Regulation of Fatty Acid Biosynthesis in the Response of Shewanella piezotolerans WP3 to Different Temperatures and Pressures J. Bacteriol., April 15, 2009; 191(8): 2574 - 2584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hosoya, S. Suzuki, K. Adachi, S. Matsuda, and H. Kasai Paramoritella alkaliphila gen. nov., sp. nov., a member of the family Moritellaceae isolated in the Republic of Palau Int J Syst Evol Microbiol, February 1, 2009; 59(2): 411 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Alexandre, M. Laranjo, J. P. W. Young, and S. Oliveira dnaJ is a useful phylogenetic marker for alphaproteobacteria Int J Syst Evol Microbiol, December 1, 2008; 58(12): 2839 - 2849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-W. Chang, S. W. Roh, K.-H. Kim, Y.-D. Nam, C. O. Jeon, H.-M. Oh, and J.-W. Bae Shewanella basaltis sp. nov., a marine bacterium isolated from black sand Int J Syst Evol Microbiol, August 1, 2008; 58(8): 1907 - 1910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kim, K. S. Baik, M. S. Kim, B.-M. Jung, T.-S. Shin, G.-H. Chung, M. S. Rhee, and C. N. Seong Shewanella haliotis sp. nov., isolated from the gut microflora of abalone, Haliotis discus hannai Int J Syst Evol Microbiol, December 1, 2007; 57(12): 2926 - 2931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Wang, F. Wang, Q. Li, and X. Xiao A Novel Filamentous Phage from the Deep-Sea Bacterium Shewanella piezotolerans WP3 Is Induced at Low Temperature J. Bacteriol., October 1, 2007; 189(19): 7151 - 7153. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
