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Int J Syst Evol Microbiol 57 (2007), 136-140; DOI  10.1099/ijs.0.64612-0
© 2007 International Union of Microbiological Societies

Nocardioides insulae sp. nov., isolated from soil

Jung-Hoon Yoon, So-Jung Kang, Choong-Hwan Lee and Tae-Kwang Oh

Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea

Correspondence
Jung-Hoon Yoon
jhyoon{at}kribb.re.kr

A Gram-positive, rod-shaped or coccoid bacterial strain, DS-51T, was isolated from a soil in Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain DS-51T grew optimally at pH 8.0 and 30 °C without NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-51T forms a distinct line of descent within the radiation enclosed by the genus Nocardioides. The chemotaxonomic properties of strain DS-51T were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on LL-2,6-diaminopimelic acid, MK-8(H4) was the predominant menaquinone and iso-C16 : 0 was the major fatty acid. The DNA G+C content was 71.1 mol%. The 16S rRNA gene sequence of strain DS-51T had similarity levels of 92.5–95.1 % with the sequences of the type strains of Nocardioides species. Strain DS-51T could be distinguished from other Nocardioides species by differences in some phenotypic characteristics. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain DS-51T represents a novel species of the genus Nocardioides, for which the name Nocardioides insulae sp. nov. is proposed. The type strain is DS-51T (=KCTC 19180T=DSM 17944T).


The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DS-51T is DQ786794.




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