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Description of Sphingosinicella xenopeptidilytica sp. nov., a -peptide-degrading species, and emended descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans

¹ Swiss Federal Institute of Aquatic Science and Technology, Eawag, CH-8600 Dübendorf, Switzerland
² Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria
³ Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany

Correspondence
Hans-Peter E. Kohler
kohler{at}eawag.ch

A Gram-negative, rod-shaped bacterium, strain 3-2W4^T, was isolated from the aeration tank of a wastewater treatment plant in Zurich and was found to have the exceptional capacity to degrade synthetic -peptides. 16S rRNA gene sequence analysis showed that strain 3-2W4^T is closely related to Sphingosinicella microcystinivorans Y2^T, but DNA–DNA hybridization experiments between these two strains revealed that they belong to two different species. The two strains displayed different fingerprints after PCR analysis using the repetitive primers BOX, ERIC and REP. Strain 3-2W4^T did not degrade microcystin, which is a characteristic trait of Sphingosinicella microcystinivorans Y2^T. Like Sphingosinicella microcystinivorans Y2^T, strain 3-2W4^T had the following characteristics: fatty acids comprising mainly C_{18 : 1}7c, summed feature 3 (C_{16 : 1}7c and/or iso-C_{15 : 0} 2-OH) and C_{16 : 0}, the presence of ubiquinone Q-10 and sym-homospermidine as the predominant polyamine compound. The polar lipid profiles of the two strains were almost identical, consisting of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and sphingoglycolipid. Strain 3-2W4^T and Sphingosinicella microcystinivorans Y2^T utilized the -peptides H-hVal-hAla-hLeu-OH and H-hAla-hLeu-OH as sole carbon and energy sources and shared -peptidyl aminopeptidase activity in common, which distinguishes them from Sphingomonas and Sphingopyxis type strains. On the basis of these results, strain 3-2W4^T represents a novel species of the genus Sphingosinicella, for which the name Sphingosinicella xenopeptidilytica sp. nov. is proposed. The type strain is 3-2W4^T (=DSM 17130^T=CCUG 52537^T). The descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans are emended.

A two-dimensional thin-layer chromatogram of the polar lipids of strain 3-2W4^T is available as a supplementary figure in IJSEM Online.

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