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Faculty of Agriculture, Yamagata University, Wakaba-machi 1-23, Tsuruoka 997-8555, Japan
Correspondence
Atsuko Ueki
uatsuko{at}tds1.tr.yamagata-u.ac.jp
A strictly anaerobic, xylanolytic bacterium, strain KB3T, isolated from rice-plant residue in flooded anoxic rice-field soil in Japan, was characterized phenotypically and phylogenetically. Cells were Gram-negative, non-motile, non-spore-forming, short to filamentous rods. Growth of the strain was remarkably stimulated by the addition of haemin to the medium. The novel strain utilized various sugars including xylan, xylose, pectin and carboxymethylcellulose and produced acetate, propionate and succinate with a small amount of malate. Propionate production was stimulated by the addition of a B-vitamin mixture or cobalamin to the medium. The novel strain was slightly acidophilic with an optimum pH 5.7–6.2 and the optimum growth temperature was 30 °C. Oxidase, catalase and nitrate-reducing activities were negative. Aesculin was hydrolysed. The major cellular fatty acids were anteiso-C15 : 0 and iso-3-OH C17 : 0. The major respiratory quinones were menaquinones MK-12(H2) and MK-13(H2). The genomic DNA G+C content was 43.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence placed the strain in the phylum Bacteroidetes. The closest related species was Prevotella bivia with a 16S rRNA gene sequence similarity of 89.5 %. Prevotella albensis and Prevotella oulorum were the next closest recognized species with sequence similarities of 89.1 %. Based on a comprehensive examination of the differences in phylogenetic, ecological, physiological and chemotaxonomic characteristics of strain KB3T and those of related species, a novel genus and species, Xylanibacter oryzae gen. nov., sp. nov., is proposed to accommodate strain KB3T. The type strain of the novel species is KB3T (=JCM 13648T=DSM 17970T).
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KB3T is AB078826.
Present address: Creative Research Initiative ‘Sousei’ (CRIS), Hokkaido University, Kita 21, Nishi 10, Kita-ku, Sapporo, 001-0021, Japan.
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  T. Nishiyama, A. Ueki, N. Kaku, K. Watanabe, and K. Ueki Bacteroides graminisolvens sp. nov., a xylanolytic anaerobe isolated from a methanogenic reactor treating cattle waste Int J Syst Evol Microbiol, August 1, 2009; 59(8): 1901 - 1907. [Abstract] [Full Text] [PDF]
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T. Nishiyama, A. Ueki, N. Kaku, and K. Ueki Clostridium sufflavum sp. nov., isolated from a methanogenic reactor treating cattle waste Int J Syst Evol Microbiol, May 1, 2009; 59(5): 981 - 986. [Abstract] [Full Text] [PDF]
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D. Suzuki, A. Ueki, A. Amaishi, and K. Ueki Desulfoluna butyratoxydans gen. nov., sp. nov., a novel Gram-negative, butyrate-oxidizing, sulfate-reducing bacterium isolated from an estuarine sediment in Japan Int J Syst Evol Microbiol, April 1, 2008; 58(4): 826 - 832. [Abstract] [Full Text] [PDF]
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A. Ueki, K. Abe, N. Kaku, K. Watanabe, and K. Ueki Bacteroides propionicifaciens sp. nov., isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms Int J Syst Evol Microbiol, February 1, 2008; 58(2): 346 - 352. [Abstract] [Full Text] [PDF]
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A. Ueki, H. Akasaka, A. Satoh, D. Suzuki, and K. Ueki Prevotella paludivivens sp. nov., a novel strictly anaerobic, Gram-negative, hemicellulose-decomposing bacterium isolated from plant residue and rice roots in irrigated rice-field soil Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1803 - 1809. [Abstract] [Full Text] [PDF]
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