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Lactobacillus siliginis sp. nov., isolated from wheat sourdough in South Korea

¹ Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea
² National University of Uzbekistan, Students Town, Tashkent 700-174, Uzbekistan

Correspondence
Sung-Taik Lee
e_stlee{at}kaist.ac.kr

The taxonomic position of two lactic-acid-producing bacterial strains, isolated from wheat sourdough in South Korea, was studied using a polyphasic approach. Phylogenetic analysis on the basis of 16S rRNA gene sequences and biochemical and physiological characteristics indicated these two strains to be members of the genus Lactobacillus. They had high 16S rRNA gene sequence similarity (98.5 %) with Lactobacillus rossiae DSM 15814^T and very low (<94.0 %) similarity with any other recognized species of the genus Lactobacillus. These two strains (designated M1-212^T and M2-236) were heterofermentative, facultatively anaerobic, Gram-positive, non-spore-forming, non-motile, short rod-shaped bacteria. The optimum growth temperature for these strains was 30 °C (no growth at 15 or 45 °C) and they were able to tolerate 5 % (w/v) NaCl. The G+C content of the genomic DNA of the two strains was 45.5 mol%, within the range of values reported for the genus Lactobacillus (32–53 mol%). The peptidoglycan was of the A3 (L-lys–D-glu–L-Ala) type. Physiological, biochemical and genotypic data, as well as results of DNA–DNA hybridization of the genomic DNA with one of the closest phylogenetic relatives, L. rossiae DSM 15814^T, indicated that the strains represent a novel species of the genus Lactobacillus, for which the name Lactobacillus siliginis sp. nov. is proposed. The type strain is M1-212^T (=KCTC 3985^T=NBRC 101315^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains M1-212^T and M2-236 are DQ168027 and DQ168028, respectively.

Figures showing cells of strain M1-212^T and PCR-generated DNA fingerprints of strains M1-212^T, M2-236 and L. rossiae DSM 15814^T are available as supplementary material in IJSEM Online.

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