| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Universidade Federal Rural do Rio de Janeiro, km 45, BR 465, C.P. 74505, Seropédica, Rio de Janeiro, Brazil
2 Embrapa Agrobiology, km 47, BR 465, C.P. 74505, Seropédica, Rio de Janeiro, Brazil
3 Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, Mexico
Correspondence
J. Caballero-Mellado
jesuscab{at}cifn.unam.mx
In a previous study, nitrogen-fixing isolates were recovered from the rhizosphere of maize and from surface-sterilized leaves of sugar cane cultivated in Rio de Janeiro, Brazil. On the basis of 16S rRNA gene sequence similarities, these isolates were identified as belonging to the genus Burkholderia, and whole-cell-protein profiles demonstrated that they are closely related to each other. In the present study, novel isolates were recovered from the roots of different sugar-cane varieties cultivated in diverse geographical regions of Brazil. Twenty-one nitrogen-fixing isolates were analysed using polyphasic taxonomy criteria, including DNA–DNA relatedness, 16S rRNA gene sequence similarities, fatty acid profiles, whole-cell-protein patterns and multilocus enzyme electrophoresis profiles, as well as morphological, physiological and biochemical characterization. The analysis confirmed that these isolates belong to a novel species within the genus Burkholderia, for which the name Burkholderia silvatlantica sp. nov. is proposed. The type strain, SRMrh-20T (=LMG 23149T=ATCC BAA-1244T), was isolated from the rhizosphere of maize var. Avantis A2345 cultivated in Seropédica, Rio de Janeiro.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains SRMrh-20T, SRCL-18, SRMrh-85 and PPCR-2 are AY965240–AY965243, respectively.
A dendrogram derived from MLEE and SDS-PAGE showing whole-cell protein profiles for the novel strains, N2-fixing Burkholderia species and B. sacchari are available as supplementary figures in IJSEM Online.
These authors contributed equally to this work.
This article has been cited by other articles:
|
|
 |
|
  L. Martinez-Aguilar, R. Diaz, J. J. Pena-Cabriales, P. Estrada-de los Santos, M. F. Dunn, and J. Caballero-Mellado Multichromosomal Genome Structure and Confirmation of Diazotrophy in Novel Plant-Associated Burkholderia Species Appl. Envir. Microbiol., July 15, 2008; 74(14): 4574 - 4579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. R. Suarez-Moreno, J. Caballero-Mellado, and V. Venturi The new group of non-pathogenic plant-associated nitrogen-fixing Burkholderia spp. shares a conserved quorum-sensing system, which is tightly regulated by the RsaL repressor Microbiology, July 1, 2008; 154(7): 2048 - 2059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Vandamme, K. Opelt, N. Knochel, C. Berg, S. Schonmann, E. De Brandt, L. Eberl, E. Falsen, and G. Berg Burkholderia bryophila sp. nov. and Burkholderia megapolitana sp. nov., moss-associated species with antifungal and plant-growth-promoting properties Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2228 - 2235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Caballero-Mellado, J. Onofre-Lemus, P. Estrada-de los Santos, and L. Martinez-Aguilar The Tomato Rhizosphere, an Environment Rich in Nitrogen-Fixing Burkholderia Species with Capabilities of Interest for Agriculture and Bioremediation Appl. Envir. Microbiol., August 15, 2007; 73(16): 5308 - 5319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Valverde, P. Delvasto, A. Peix, E. Velazquez, I. Santa-Regina, A. Ballester, C. Rodriguez-Barrueco, C. Garcia-Balboa, and J. M. Igual Burkholderia ferrariae sp. nov., isolated from an iron ore in Brazil. Int J Syst Evol Microbiol, October 1, 2006; 56(Pt 10): 2421 - 2425. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
