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Novel nitrogen-fixing Acetobacter nitrogenifigens sp. nov., isolated from Kombucha tea

Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700032, West Bengal, India

Correspondence
Ratan Gachhui
ratangachhui{at}yahoo.com

The four nitrogen-fixing bacteria so far described in the family Acetobacteraceae belong to the genera Gluconacetobacter and Acetobacter. Nitrogen-fixing bacterial strain RG1^T was isolated from Kombucha tea and, based on the phylogenetic analysis of 16S rRNA gene sequence which is supported by a high bootstrap value, was found to belong to the genus Acetobacter. Strain RG1^T differed from Acetobacter aceti, the nearest member with a 16S rRNA gene sequence similarity of 98.2 %, and type strains of other Acetobacter species with regard to several characteristics of growth features in culture media, growth in nitrogen-free medium, production of -pyrone from glucose and dihydroxyacetone from glycerol. Strain RG1^T utilized maltose, glycerol, sorbitol, fructose, galactose, arabinose and ethanol, but not methanol as a carbon source. These results, along with electrophoretic mobility patterns of nine metabolic enzymes, suggest that strain RG1^T represents a novel nitrogen-fixing species. The ubiquinone present was Q-9 and DNA G+C content was 64.1 mol%. Strain RG1^T exhibited a low value of 2–24 % DNA–DNA relatedness to the type strains of related acetobacters, which placed it as a separate taxon. On the basis of this data, the name Acetobacter nitrogenifigens sp. nov. is proposed, with the type strain RG1^T (=MTCC 6912^T=LMG 23498^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and nifH gene sequences of strain RG1^T are AY669513 and AY952470, respectively.

A MLEE dendrogram showing the relationships between Acetobacter nitrogenifigens sp. nov. and type strains of closely related Acetobacter species is available as a supplementary figure in IJSEM Online.

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