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1 Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok 10330, Thailand
2 Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan
Correspondence
Somboon Tanasupawat
Somboon.T{at}chula.ac.th
Fifteen strains of extremely halophilic bacteria were isolated from fish sauce (nam-pla) collected in Thailand at various stages of the fish-fermentation process. The isolates were strictly aerobic, spore-forming, Gram-positive rods. They grew optimally in the presence of 20–26 % NaCl. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The DNA G+C content was 42.1–43.1 mol%. On the basis of the 16S rRNA gene sequence, a representative strain, PS11-2T, was found to be closely related to Lentibacillus juripiscarius JCM 12147T (97.3 % similarity). The 15 strains were included in the same species on the basis that the levels of DNA–DNA relatedness with strain PS11-2T were greater than 70 %. They could be distinguished from L. juripiscarius and other Lentibacillus species on the basis of several phenotypic characteristics and low levels of DNA–DNA relatedness (
19.4 %). Therefore, the strains represent a novel species of the genus Lentibacillus, for which the name Lentibacillus halophilus sp. nov. is proposed. The type strain is PS11-2T (=JCM 12149T=TISTR 1549T=PCU 240T).
A scanning electron micrograph of sporulating cells of strain PS11-2T, a thin-layer chromatogram of polar lipids and details of the cellular fatty acids and levels of DNA–DNA relatedness of the novel strains and related taxa are available as supplementary material in IJSEM Online.
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