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1 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
2 Institut Mediterrani d'Estudis Avancats (CSIC-UIB), E-07190 Esporles, Mallorca, Spain
3 Bundeswehr Institute of Microbiology, D-80937 Munich, Germany
4 Culture Collection University of Göteborg, Department of Clinical Bacteriology, S-41346 Göteborg, Sweden
5 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria
Correspondence
Peter Kämpfer
peter.kaempfer{at}agrar.uni-giessen.de
Two Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacteria (CCUG 46016T and CCUG 33852T), isolated from a knee aspirate of a 66-year-old man and an industrial glue, respectively, were studied for their taxonomic position. On the basis of chemotaxonomic data [i.e. major ubiquinone (Q-10), major polar lipids (phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine) and major fatty acids (C18 : 1
7c and C19 : 0 cyclo 8c)] and 16S rRNA gene sequence similarity, both strains belong to the Alphaproteobacteria. The presence of spermidine and putrescine as the predominant polyamines in CCUG 46016T were in agreement with its phylogenetic affiliation in the vicinity of the genus Ochrobactrum. 16S rRNA gene sequence similarities between both strains and established species within the genera Bartonella, Ochrobactrum and Brucella were less than 95 %. Although both organisms showed highest 16S rRNA gene sequence similarity to members of Brucella, phenotypic features (including chemotaxonomic features) were more like those of members of the genus Ochrobactrum. Sequence comparison of the recA genes confirmed the separate phylogenetic position of the two strains. On the basis of DNA–DNA pairing results and physiological and biochemical data, the two strains can be clearly differentiated from each other and from all known Ochrobactrum species. It is evident that these organisms represent two novel species in a new genus, Pseudochrobactrum gen. nov., for which the names Pseudochrobactrum asaccharolyticum sp. nov. (the type species, type strain CCUG 46016T=CIP 108977T) and Pseudochrobactrum saccharolyticum sp. nov. (type strain CCUG 33852T=CIP 108976T) are proposed.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains CCUG 46016T and CCUG 33852T are AM180485 and AM180484, respectively, and those of the partial recA sequences of strains CCUG 46016T and CCUG 33852T are AM118081 and AM118082.
A multiple sequence alignment of partial recA gene sequences and a two-dimensional TLC of the polar lipids of CCUG 46016T are available as supplementary material in IJSEM Online.
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