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Lactobacillus cypricasei Lawson et al. 2001 is a later heterotypic synonym of Lactobacillus acidipiscis Tanasupawat et al. 2000

¹ Laboratory of Microbiology, Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium
² BCCM^TM/LMG Bacteria Collection, Ghent University, K.L. Ledeganckstraat 35, Ghent 9000, Belgium

Correspondence
Sabri M. Naser
Sabri.Naser{at}Ugent.be

The applicability of a multilocus sequence analysis (MLSA)-based identification system for lactobacilli was evaluated. Two housekeeping genes that code for the phenylalanyl-tRNA synthase -subunit (pheS) and RNA polymerase -subunit (rpoA) were sequenced and analysed for members of the Lactobacillus salivarius species group. The type strains of Lactobacillus acidipiscis and Lactobacillus cypricasei were investigated further using a third gene that encodes the -subunit of ATP synthase (atpA). The MLSA data revealed close relatedness between L. acidipiscis and L. cypricasei, with 99.8–100 % pheS, rpoA and atpA gene sequence similarities. Comparison of the 16S rRNA gene sequences of the type strains of the two species confirmed the close relatedness (99.8 % gene sequence similarity) between the two taxa. Similar phenotypes and high DNA–DNA binding values in the range of 84 to 97.5 % confirmed that L. acidipiscis and L. cypricasei are synonymous species. On the basis of the present study, it is proposed that Lactobacillus cypricasei is a later heterotypic synonym of Lactobacillus acidipiscis.

The GenBank/EMBL/DDBJ accession numbers for the partial pheS gene sequences for strains LMG 19820^T, LMG 21592^T, LMG 23135, CCUG 42959, CCUG 42960, CCUG 42962, LMG 9843^T, LMG 14189^T, LMG 22087^T, LMG 21748^T, LMG 9186^T, LMG 10753^T, LMG 10756^T, LMG 21593^T, LMG 9477^T, LMG 6899^T and LMG 6903^T are AM087762, AM087687, AM168426–AM168429, AM087679, AM087760, AM087717, AM087740, AM087734, AM087737, AM087756, AM087708, AM087721, AM087746 and AM168425. Those for the rpoA partial gene sequences for strains LMG 19820^T, LMG 21592^T, LMG 23135, CCUG 42959, CCUG 42960, LMG 9186^T and LMG 14189^T are AM087849, AM087784, AM168431–AM168433, AM087831 and AM087801, respectively. Those for the atpA partial gene sequences for strains LMG 19820^T and LMG 21592^T are AM168424–AM168423, respectively.

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