HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Tables and Figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sakamoto, M. Articles by Benno, Y. Search for Related Content PubMed PubMed Citation Articles by Sakamoto, M. Articles by Benno, Y. Agricola Articles by Sakamoto, M. Articles by Benno, Y.

Reclassification of Bacteroides distasonis, Bacteroides goldsteinii and Bacteroides merdae as Parabacteroides distasonis gen. nov., comb. nov., Parabacteroides goldsteinii comb. nov. and Parabacteroides merdae comb. nov.

Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan

Correspondence
Mitsuo Sakamoto
sakamoto{at}jcm.riken.jp

The characteristics of three Bacteroides species, Bacteroides distasonis, Bacteroides goldsteinii and Bacteroides merdae, were examined. 16S rRNA gene sequence analysis showed that B. distasonis, B. goldsteinii and B. merdae should not be classified as species within the genus Bacteroides. Although B. distasonis, B. goldsteinii and B. merdae were phylogenetically related to Tannerella forsythensis, the ratios of anteiso-C_{15 : 0} to iso-C_{15 : 0} in whole-cell methanolysates of the three species were different from that of T. forsythensis. In addition, whereas the major menaquinones of T. forsythensis were MK-10 and MK-11, the major menaquinones of B. distasonis, B. goldsteinii and B. merdae were MK-9 and MK-10. The three species were phenotypically similar to Bacteroides sensu stricto, but phylogenetically distinct. Furthermore, B. distasonis, B. goldsteinii and B. merdae could be differentiated from Bacteroides sensu stricto (predominant menaquinones: MK-10 and MK-11) by the menaquinone composition. This is an important chemotaxonomic characteristic of the three species. On the basis of these data, a novel genus, Parabacteroides gen. nov., is proposed for B. distasonis, B. goldsteinii and B. merdae, with three species, Parabacteroides distasonis gen. nov., comb. nov. (the type species), Parabacteroides goldsteinii comb. nov. and Parabacteroides merdae comb. nov. The type strains of P. distasonis, P. goldsteinii and P. merdae are JCM 5825^T (=CCUG 4941^T=DSM 20701^T=ATCC 8503^T), JCM 13446^T (=CCUG 48944^T) and JCM 9497^T (=CCUG 38734^T=ATCC 43184^T), respectively.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of P. distasonis strains JCM 5825^T, JCM 13400, JCM 13401, JCM 13402, JCM 13403 and JCM 13404 and P. merdae strains JCM 9497^T and JCM 13405 are AB238922–AB238929, respectively.

The cellular fatty acid content and biochemical characteristics of Parabacteroides distasonis gen. nov., comb. nov. and Parabacteroides merdae comb. nov. and a phylogenetic tree based on sequences of 16S–23S rRNA gene ITS regions are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				H. M. Wexler Bacteroides: the Good, the Bad, and the Nitty-Gritty Clin. Microbiol. Rev., October 1, 2007; 20(4): 593 - 621. [Abstract] [Full Text] [PDF]

				M. Sakamoto, M. Kitahara, and Y. Benno Parabacteroides johnsonii sp. nov., isolated from human faeces Int J Syst Evol Microbiol, February 1, 2007; 57(2): 293 - 296. [Abstract] [Full Text] [PDF]

				M. Sakamoto, P. T. N. Lan, and Y. Benno Barnesiella viscericola gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from chicken caecum Int J Syst Evol Microbiol, February 1, 2007; 57(2): 342 - 346. [Abstract] [Full Text] [PDF]