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1 Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium
2 Department of Biochemistry, Physiology and Microbiology, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium
3 CODA–CERVA–VAR, Groeselenberg 99, B-1180 Brussels, Belgium
Correspondence
K. Van den Bulck
kvdbulck{at}prdbe.jnj.com
A Gram-negative, microaerophilic helical rod, isolated from the gastric mucosa of a dog and designated strain JKM4T, was subjected to a polyphasic taxonomic study. The tightly coiled organism, measuring 10–18 µm long and up to 1 µm wide, was motile by means of multiple sheathed flagella located at both ends of the cell and by a periplasmic fibril running along the external side of the helix. Strain JKM4T grew preferably on biphasic culture plates or on very moist agar. Coccoid forms predominated in cultures older than 4 days as well as in growth obtained on dry agar plates. The strain grew at 30 and 37 °C, but not at 25 or 42 °C and exhibited urease, oxidase and catalase activities. On the basis of 16S rRNA gene sequence analysis, the novel isolate was identified as a member of the genus Helicobacter and showed > 97 % similarity to Helicobacter felis, Helicobacter bizzozeronii and Helicobacter salomonis, three species previously isolated from the canine gastric mucosa. Protein profiling of strain JKM4T using SDS-PAGE revealed a pattern different from those of other Helicobacter species of mammalian gastric origin and from Helicobacter canis. Additionally, the urease gene sequence of strain JKM4T was different from those of urease genes of H. felis, H. bizzozeronii, H. salomonis and ‘Candidatus Helicobacter heilmannii’. It is thus proposed that strain JKM4T (=LMG 23188T) represents a novel species within this genus, Helicobacter cynogastricus sp. nov.
A phylogenetic tree based on 16S rRNA gene sequences for 27 Helicobacter strains and TEM images of strain JKM4T are available as supplementary figures in IJSEM Online. A supplementary table detailing the 16S rRNA gene sequence similarity between Helicobacter strains is also available.
Present address: Janssen Pharmaceutica N.V., Global Preclinical Development, Turnhoutseweg 30, B-2340 Beerse, Belgium.
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