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Pseudonocardia tetrahydrofuranoxydans sp. nov.

¹ Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany
² Institut für Mikrobiologie, Martin-Luther-Universität Halle, D-06099 Halle, Germany

Correspondence
Peter Kämpfer
peter.kaempfer{at}agrar.uni-giessen.de

A Gram-positive, rod-shaped, non-endospore-forming but mycelium-forming actinobacterium (strain K1^T) was isolated from an enrichment culture containing tetrahydrofuran (THF) as the sole source of carbon. On the basis of its G+C content (71.3 mol%) and of 16S rRNA gene sequence similarity studies, strain K1^T was shown to belong to the family Pseudonocardiaceae, most closely related to Pseudonocardia hydrocarbonoxydans (99.3 %), P. benzenivorans (98.8 %) and P. sulfidoxydans (98.3 %). The 16S rRNA gene sequence similarity to other Pseudonocardia species was less than 97 %. Chemotaxonomic data [major menaquinone MK-8(H₄); major fatty acids C_{16 : 0} iso, C_{15 : 0} iso and C_{17 : 1}6c] supported the affiliation of strain K1^T to the genus Pseudonocardia. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain K1^T from the three species P. benzenivorans, P. sulfidoxydans and P. hydrocarbonoxydans, although all four organisms utilized THF. Strain K1^T represents a novel species, for which the name Pseudonocardia tetrahydrofuranoxydans sp. nov. is proposed, with the type strain K1^T (=DSM 44239^T=CIP 109050^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain K1^T is AJ249200.

A maximum-parsimony tree based on 16S rRNA gene sequences and details of the fatty acid composition of strain K1^T and related type strains are available as supplementary material in IJSEM Online.

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