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Paenibacillus pasadenensis sp. nov. and Paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility

¹ Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, 89-2; Biotechnology and Planetary Protection Group, 4800, Oak Grove Dr., Pasadena, CA 91109, USA
² National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, 236-8648, Japan

Correspondence
Shariff Osman
sosman{at}jpl.nasa.gov

Two novel spore-forming, Gram-positive, mesophilic, heterotrophic bacteria representing two novel species were isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) at Pasadena, CA, USA. The incidence of similar strains was examined by screening the growing collection of isolates (400 strains) obtained from the JPL-SAF using species-specific PCR primer sets designed from the 16S rRNA gene sequences of strains SAFN-016^T and SAFN-007^T. Phylogenetic analysis of 16S rRNA gene sequences placed these novel isolates within the genus Paenibacillus. Two strains, SAFN-016^T and SAFN-125, shared 98 % 16S rRNA gene sequence similarity with Paenibacillus timonensis and 97 % similarity with Paenibacillus macerans. Strain SAFN-007^T showed 95.2 % 16S rRNA gene sequence similarity with Paenibacillus kobensis, its nearest phylogenetic neighbour. The results of DNA–DNA hybridization, physiological tests and biochemical analysis allowed genotypic and phenotypic differentiation of the isolates from currently recognized Paenibacillus species. Strain SAFN-007^T and strains SAFN-016^T and SAFN-125 are representatives of two separate novel species, for which the names Paenibacillus pasadenensis sp. nov. (type strain SAFN-007^T=ATCC BAA-1211^T=NBRC 101214^T) and Paenibacillus barengoltzii sp. nov. (type strain SAFN-016^T=ATCC BAA-1209^T=NBRC 101215^T) are proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of the Paenibacillus strains investigated in this study are given in Table 1.

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