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Tetrathiobacter mimigardefordensis sp. nov., isolated from compost, a betaproteobacterium capable of utilizing the organic disulfide 3,3'-dithiodipropionic acid

Jan Hendrik Wübbeler¹, Tina Lütke-Eversloh^1,, Stefanie Van Trappen², Peter Vandamme² and Alexander Steinbüchel¹

¹ Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, Corrensstraße 3, 48149 Münster, Germany
² Laboratory of Microbiology, Department of Biochemistry, Physiology and Microbiology, University of Ghent, K. L. Ledeganckstraat 35, 9000 Ghent, Belgium

Correspondence
Alexander Steinbüchel
steinbu{at}uni-muenster.de

In this study, a novel betaproteobacterium, strain DPN7^T, was isolated under mesophilic conditions from compost because of its capacity to utilize the organic disulfide 3,3'-dithiodipropionic acid. Analysis of the 16S rRNA gene sequence of strain DPN7^T revealed 98.5 % similarity to that of Tetrathiobacter kashmirensis LMG 22695^T. Values for sequence similarity to members of the genera Alcaligenes, Castellaniella and Taylorella, the nearest neighbours of the genus Tetrathiobacter, were about 95 % or less. The DNA G+C content of strain DPN7^T was 55.1 mol%. The level of DNA–DNA hybridization between strain DPN7^T and T. kashmirensis LMG 22695^T was 41 %, whereas it was much lower between strain DPN7^T and Alcaligenes faecalis LMG 1229^T (7 %) or Castellaniella defragrans LMG 18538^T (5 %). This genotypic divergence was supported by differences in biochemical and chemotaxonomic characteristics. For this reason, and because of the differences in the protein and fatty acid profiles, strain DPN7^T should be classified within a novel species of Tetrathiobacter, for which the name Tetrathiobacter mimigardefordensis sp. nov. is proposed. The type strain is strain DPN7^T (=DSM 17166^T=LMG 22922^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DPN7^T is AY880023.

A phase-contrast micrograph of cells of strain DPN7^T is available as supplementary material in IJSEM Online.

Present addresss: Massachusetts Institute of Technology, Department of Chemical Engineering, 77 Massachusetts Avenue 56-422, Cambridge, MA 02139, USA.

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