HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bakermans, C. Articles by Tiedje, J. M. Search for Related Content PubMed PubMed Citation Articles by Bakermans, C. Articles by Tiedje, J. M. Agricola Articles by Bakermans, C. Articles by Tiedje, J. M.

Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost

¹ Center for Microbial Ecology and NASA Astrobiology Institute, Michigan State University, East Lansing, MI 48824, USA
² Department of Biology, University of Puerto Rico at Humacao, Humacao, PR 00791, Puerto Rico
³ Department of Food Science, North Carolina State University, Raleigh, NC 27695, USA
⁴ Institute of Physico-chemical and Biological Problems in Soil Science, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation

Correspondence
Corien Bakermans
bakerm16{at}msu.edu

Three Gram-negative, non-motile, non-pigmented, oxidase-positive coccobacilli capable of growth at temperatures from –10 to 30 °C and salinities of 0 to 1.7 M NaCl were isolated from Siberian permafrost and characterized. Both 16S rRNA and gyrB gene sequencing studies placed the isolates in the Gammaproteobacteria within the genus Psychrobacter. However, with higher bootstrap values and reproducible tree topologies, gyrB represented a more reliable phylogenetic marker for the taxonomy of Psychrobacter species. DNA–DNA hybridization data supported gyrB tree topologies and established two relatedness groups within the three isolates; neither of these groups was related at the species level to any previously described Psychrobacter species. The two groups of isolates could be differentiated phenotypically from 13 previously described Psychrobacter species using API strips. These results support the existence of two novel species of Psychrobacter, for which we propose the names Psychrobacter cryohalolentis sp. nov. (type strain K5^T=DSM 17306^T=VKM B-2378^T) and Psychrobacter arcticus sp. nov. (type strain 273-4^T=DSM 17307^T=VKM B-2377^T).

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of isolates K5^T, 237-4^T and 215-51 are AY660685, AY444822 and AY444823, and those for the gyrB sequences obtained in this study are DQ143914–DQ143928 and CP000082.

This article has been cited by other articles:

				L. A. Romanenko, N. Tanaka, G. M. Frolova, and V. V. Mikhailov Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan Int J Syst Evol Microbiol, June 1, 2009; 59(6): 1480 - 1486. [Abstract] [Full Text] [PDF]

				P. W. Bergholz, C. Bakermans, and J. M. Tiedje Psychrobacter arcticus 273-4 Uses Resource Efficiency and Molecular Motion Adaptations for Subzero Temperature Growth J. Bacteriol., April 1, 2009; 191(7): 2340 - 2352. [Abstract] [Full Text] [PDF]

				P. Amato and B. C. Christner Energy Metabolism Response to Low-Temperature and Frozen Conditions in Psychrobacter cryohalolentis Appl. Envir. Microbiol., February 1, 2009; 75(3): 711 - 718. [Abstract] [Full Text] [PDF]

				J. Song, Y.-J. Choo, and J.-C. Cho Perlucidibaca piscinae gen. nov., sp. nov., a freshwater bacterium belonging to the family Moraxellaceae Int J Syst Evol Microbiol, January 1, 2008; 58(1): 97 - 102. [Abstract] [Full Text] [PDF]