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Azospirillum melinis sp. nov., a group of diazotrophs isolated from tropical molasses grass

¹ College of Resources and Environment, South China Agricultural University, Guangzhou 510642, China
² Provincial Key Lab of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
³ College of Life Science, South China Agricultural University, Guangzhou 510642, China
⁴ Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D. F. 11340, Mexico

Correspondence
Zhiyuan Tan
zytan{at}scau.edu.cn

Fifteen bacterial strains isolated from molasses grass (Melinis minutiflora Beauv.) were identified as nitrogen-fixers by using the acetylene-reduction assay and PCR amplification of nifH gene fragments. These strains were classified as a unique group by insertion sequence-PCR fingerprinting, SDS-PAGE protein patterns, DNA–DNA hybridization, 16S rRNA gene sequencing and morphological characterization. Phylogenetic analysis of the 16S rRNA gene indicated that these diazotrophic strains belonged to the genus Azospirillum and were closely related to Azospirillum lipoferum (with 97.5 % similarity). In all the analyses, including in addition phenotypic characterization using Biolog MicroPlates and comparison of cellular fatty acids, this novel group was found to be different from the most closely related species, Azospirillum lipoferum. Based on these data, a novel species, Azospirillum melinis sp. nov., is proposed for these endophytic diazotrophs of M. minutiflora, with TMCY 0552^T (=CCBAU 5106001^T=LMG 23364^T=CGMCC 1.5340^T) as the type strain.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains TMCY 243, TMCY 244, TMCY 0552^T and TMCY 41 are DQ022956–DQ022959, respectively.

IS-PCR fingerprinting patterns of the diazotrophic isolates from molasses grass and Azospirillum lipoferum DSM 1691^T, and cellular fatty acid profiles of and data on the utilization of carbon sources by Azospirillum melinis sp. nov. TMCY 0552^T and Azospirillum lipoferum DSM 1691^T are available as supplementary material in IJSEM Online.

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