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Emticicia oligotrophica gen. nov., sp. nov., a new member of the family ‘Flexibacteraceae’, phylum Bacteroidetes

Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India

Correspondence
T. Chakrabarti
tapan{at}imtech.res.in

An aquatic bacterium, strain GPTSA100-15^T, was isolated on nutritionally poor medium TSBA100 (tryptic soy broth diluted 100 times and solidified with 1.5 % agarose) and characterized using a polyphasic approach. The isolate was unable to grow on commonly used nutritionally rich media such as tryptic soy agar, nutrient agar and Luria–Bertani agar. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was affiliated with the family ‘Flexibacteraceae’ in the phylum Bacteroidetes. Phylogenetically, it showed closest similarity (94.0 %) with an uncultured bacterial clone, HP1A92, detected in a sludge microbial community. Among the culturable bacteria, the isolate had highest 16S rRNA gene sequence similarity with Leadbetterella byssophila 4M15^T (87.8 %). Sequence similarities with other members of the phylum Bacteroidetes were less than 85 %. The fatty acid profile of the isolate grown on TSBA100 indicated that the major fatty acid was iso-C_{15 : 0}, which is also present in many members of the family ‘Flexibacteraceae’. Cells of strain GPTSA100-15^T are Gram-negative, strictly aerobic rods. The DNA G+C content of the isolate is 36.9 mol%. Results of phenotypic, chemotaxonomic and phylogenetic analyses clearly indicate that strain GPTSA100-15^T represents a new genus within the family ‘Flexibacteraceae’; the name Emticicia gen. nov. is proposed for the genus, with Emticicia oligotrophica sp. nov. as the type species. The type strain of Emticicia oligotrophica is GPTSA100-15^T (=MTCC 6937^T=DSM 17448^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain GPTSA100-15^T is AY904352.

An extended phylogenetic tree based on 16S rRNA gene sequences of members of the phylum Bacteroidetes is available as supplementary material in IJSEM Online.

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