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Azonexus caeni sp. nov., a denitrifying bacterium isolated from sludge of a wastewater treatment plant

¹ Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China
² Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea

Correspondence
Sung-Taik Lee
e_stlee{at}kaist.ac.kr

A polyphasic taxonomic study was carried out to determine the taxonomic position of a newly isolated denitrifying bacterium, designated Slu-05^T, which had been isolated from sludge from the main aerobic treatment tanks of a municipal sewage treatment plant. Phylogenetic analysis based on comparative 16S rRNA gene sequencing indicated that strain Slu-05^T was closely related to Azonexus fungiphilus LMG 19178^T (96.4 % sequence similarity), the sole species in the genus Azonexus. Strain Slu-05^T comprised Gram-negative, motile, non-spore-forming and slightly curved rods. The predominant respiratory lipoquinone was Q-8. The major fatty acids were C_{16 : 1}7c, C_{16 : 0}, C_{18 : 1} isomers and C_{10 : 0} 3-OH. The G+C content of the genomic DNA was 65.6 mol%. The results of DNA–DNA hybridization (15.6 %) together with phenotypic determination showed that strain Slu-5^T could be distinguished from A. fungiphilus. Moreover, some phenotypic properties concerning enzyme activity, the substrates utilized as carbon sources and growth conditions distinguish strain Slu-5^T from A. fungiphilus. On the basis of the results obtained in this study, Slu-05^T (=DSM 17719^T=KCTC 12530^T=CCBAU 10199^T) is the type strain of a novel species of Azonexus, for which the name Azonexus caeni sp. nov. is proposed.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strain Slu-05^T and the partial nifH gene sequences of strain Slu-05^T and Azonexus fungiphilus LMG 19178^T are AB166882, DQ029203 and DQ029204, respectively.

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