HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Figure Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Xie, C.-H. Articles by Yokota, A. Search for Related Content PubMed PubMed Citation Articles by Xie, C.-H. Articles by Yokota, A. Agricola Articles by Xie, C.-H. Articles by Yokota, A.

Sphingomonas azotifigens sp. nov., a nitrogen-fixing bacterium isolated from the roots of Oryza sativa

Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo 113-0032, Japan

Correspondence
Cheng-Hui Xie
aa37116{at}mail.ecc.u-tokyo.ac.jp

Three yellow-pigmented strains associated with rice plants were characterized by using a polyphasic approach. The nitrogen-fixing abilities of these strains were confirmed by acetylene reduction assay and nifH gene detection. The three strains were found to be very closely related, with 99·9 % 16S rRNA gene sequence similarity and greater than 70 % DNA–DNA hybridization values, suggesting that the three strains represent a single species. 16S rRNA gene sequence analysis indicated that the strains were closely related to Sphingomonas trueperi, with 99·5 % similarity. The chemotaxonomic characteristics (G+C content of the DNA of 68·0 mol%, ubiquinone Q-10 system, 2-OH as the only hydroxy fatty acid and homospermidine as the sole polyamine) were similar to those of members of the genus Sphingomonas. Based on DNA–DNA hybridization values and physiological characteristics, the three novel strains could be differentiated from other recognized species of the genus Sphingomonas. The name Sphingomonas azotifigens sp. nov. is proposed to accommodate these bacterial strains; the type strain is Y39^T (=NBRC 15497^T=IAM 15283^T=CCTCC AB205007^T).

A neighbour-joining tree based on nifH gene sequences showing the relationships between strain Y39^T and other nitrogen-fixing bacteria is available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				S. L. Addison, S. M. Foote, N. M. Reid, and G. Lloyd-Jones Novosphingobium nitrogenifigens sp. nov., a polyhydroxyalkanoate-accumulating diazotroph isolated from a New Zealand pulp and paper wastewater Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2467 - 2471. [Abstract] [Full Text] [PDF]

				L. A. Romanenko, M. Uchino, G. M. Frolova, N. Tanaka, N. I. Kalinovskaya, N. Latyshev, and V. V. Mikhailov Sphingomonas molluscorum sp. nov., a novel marine isolate with antimicrobial activity Int J Syst Evol Microbiol, February 1, 2007; 57(2): 358 - 363. [Abstract] [Full Text] [PDF]