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Int J Syst Evol Microbiol 56 (2006), 873-881; DOI  10.1099/ijs.0.64050-0
© 2006 International Union of Microbiological Societies

Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov.

Dania Richter1, Danièle Postic2, Natacha Sertour2, Ian Livey3, Franz-Rainer Matuschka1 and Guy Baranton2

1 Charité Universitätsmedizin Berlin, 12249 Berlin, Germany
2 Institut Pasteur, 75724 Paris, France
3 Baxter Vaccine AG, A-2304 Orth/Donau, Austria

Correspondence
Dania Richter
drichter{at}charite.de

To evaluate multilocus sequence analysis (MLSA) for taxonomic purposes in the delineation of species within Borrelia burgdorferi sensu lato, seven relevant loci of various strains for which extensive DNA–DNA reassociation data were available were sequenced. MLSA delineation proved to be fully concordant with conventional methods. Our analysis confirmed the delineation of a novel species, Borrelia spielmanii sp. nov., previously known as ‘Borrelia spielmani’ Richter et al. 2004, with strain PC-Eq17N5T (=DSM 16813T=CIP 108855T) as the type strain.


Abbreviations: MLEE, multilocus enzyme electrophoresis; MLSA, multilocus sequence analysis; MLST, multilocus sequence typing; WDDH, whole DNA–DNA hybridization

Published online ahead of print on 9 December 2005 as DOI 10.1099/ijs.0.64050-0.

The GenBank/EMBL/DDBJ accession numbers for the fla, groEL, hbb, ospA, recA, rrs and rrf–rrl intergenic spacer gene sequences of Borrelia spielmanii sp. nov. PC-Eq17N5T are DQ133508, DQ133511, DQ133514, DQ133517, DQ133520, DQ133523 and DQ133526, respectively. Accession numbers for other gene sequences obtained in this study are given in Supplementary Table S2 in IJSEM Online.

Tables detailing similarity values for concatenated sequences of species within Borrelia burgdorferi s.l. and GenBank accession numbers for gene sequences derived in this study are available as supplementary material in IJSEM Online.




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