HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Slepova, T. V. Articles by Bonch-Osmolovskaya, E. A. Search for Related Content PubMed PubMed Citation Articles by Slepova, T. V. Articles by Bonch-Osmolovskaya, E. A. Agricola Articles by Slepova, T. V. Articles by Bonch-Osmolovskaya, E. A.

Carboxydocella sporoproducens sp. nov., a novel anaerobic CO-utilizing/H₂-producing thermophilic bacterium from a Kamchatka hot spring

¹ Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60 Let Oktyabrya, 7/2, 117811 Moscow, Russia
² Lomonosov Moscow State University, Biology faculty, Vorob'evy gory, 119899 Moscow, Russia
³ Institute of Volcanology and Seismology, Far-East Division Russian Academy of Sciences, Piip Boulevard, 9, 683006 Petropavlovsk-Kamchatsky, Russia

Correspondence
Tatiana V. Slepova
slepysh{at}gmail.com

A novel anaerobic, thermophilic, CO-utilizing bacterium, strain Kar^T, was isolated from a hot spring of Karymskoe Lake, Kamchatka Peninsula. The cells of the novel isolate were Gram-positive, spore-forming, short rods. The bacterium grew chemolithoautotrophically on CO, producing equimolar quantities of H₂ and CO₂ (according to the equation CO + H₂O CO₂ + H₂), and in the absence of CO, under N₂ in the gas phase, chemoorganoheterotrophically with yeast extract, sucrose or pyruvate. Growth was observed in the temperature range 50–70 °C, with an optimum at 60 °C, and in the pH range 6·2–8·0, with an optimum at pH 6·8. The micro-organism did not grow on solid media; it was able to grow only in semi-solid medium containing 0·5 % agar. The generation time under optimal conditions for chemolithoautotrophic growth was 1 h. The G+C content of the DNA was 46·5±1 mol%. Growth was completely inhibited by penicillin, novobiocin, streptomycin, kanamycin and neomycin. Analysis of the 16S rRNA gene sequence showed that the isolate should be assigned to the genus Carboxydocella. On the basis of the results of DNA–DNA hybridization and morphological and physiological analyses, strain Kar^T represents a novel species of the genus Carboxydocella, for which the name Carboxydocella sporoproducens sp. nov. is proposed. The type strain is Kar^T (=DSM 16521^T=VKM B-2358^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Kar^T is AY673988.

This article has been cited by other articles:

				H. S. Lee, S. G. Kang, S. S. Bae, J. K. Lim, Y. Cho, Y. J. Kim, J. H. Jeon, S.-S. Cha, K. K. Kwon, H.-T. Kim, et al. The Complete Genome Sequence of Thermococcus onnurineus NA1 Reveals a Mixed Heterotrophic and Carboxydotrophic Metabolism J. Bacteriol., November 15, 2008; 190(22): 7491 - 7499. [Abstract] [Full Text] [PDF]