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Halovivax asiaticus gen. nov., sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China

¹ Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain
² Noda Institute for Scientific Research, 399 Noda, Noda-shi, Chiba-ken 278-0037, Japan
³ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China
⁴ Department of Biotechnology, University of the Western Cape, Bellville 7535, Cape Town, South Africa
⁵ Genencor International BV, Archimedesweg 30, 2333 CN Leiden, The Netherlands
⁶ Department of Infection, Immunity and Inflammation, University of Leicester, Leicester LE1 9HN, UK

Correspondence
A. Ventosa
ventosa{at}us.es

Strain EJ-46^T, a novel pleomorphic, aerobic, extremely halophilic member of the Archaea was isolated from sediment of the saline Lake Ejinor, in Inner Mongolia, China. This organism was neutrophilic and required at least 15 % (2·5 M) NaCl for growth. MgCl₂ was not required. The isolate was able to grow at pH 6·0–9·0. Optimum growth occurred in media containing 20 % (3·4 M) NaCl at pH 7·0–7·5. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, derived from both C₂₀C₂₀ and C₂₀C₂₅ glycerol diethers. Four glycolipids were detected, one of which may be novel. The DNA G+C content was 60·3 mol%. 16S rRNA gene analysis revealed that strain EJ-46^T was a member of the phylogenetic group defined by the family Halobacteriaceae, and the highest 16S rRNA gene similarity values of 94·9 and 94·8 % were obtained with the haloalkaliphilic species of the genus Natronococcus, Natronococcus occultus and Natronococcus amylolyticus, respectively. Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the novel isolate should be classified as representing a new genus and species, for which the name Halovivax asiaticus gen. nov., sp. nov. is proposed. The type strain is EJ-46^T (=CGMCC 1.4248^T=CECT 7098^T).

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain EJ-46^T is AM039978.

TLC of polar lipids extracted from Halovivax asiaticus gen. nov., sp. nov. and some other haloarchaea are available as supplementary material in IJSEM Online.

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