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Ethanoligenens harbinense gen. nov., sp. nov., isolated from molasses wastewater

School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China

Correspondence
Nanqi Ren
rnq{at}hit.edu.cn or
ixdf{at}yahoo.com.cn

Two strictly anaerobic bacterial strains (YUAN-3^T and X-29) were isolated from anaerobic activated sludge of molasses wastewater in a continuous stirred-tank reactor. The strains were Gram-positive, non-spore-forming, mesophilic and motile. Cells were regular rods (0·4–0·8x1·5–8·0 µm) and occurred singly, in pairs and sometimes in chains of up to eight. Autoaggregative and autofluorescent growth of strain YUAN-3^T and non-aggregative growth of strain X-29 were observed at 20–44 °C and pH 3·5–9·0. Both strains hydrolysed gelatin and aesculin and fermented several kinds of mono-, di- and oligosaccharides. Fermentation end products formed from glucose were acetate, ethanol, hydrogen and carbon dioxide. The predominant cellular fatty acids were the branched-chain fatty acids iso-C_{16 : 0} (44·18 %) and iso-C_{12 : 0} (26·67 %). The DNA G+C contents of strains YUAN-3^T and X-29 were 47·8 and 49·0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represent a novel phyletic sublineage within the Clostridium cellulosi rRNA cluster, with <92 % 16S rRNA gene sequence similarity to currently known species. On the basis of polyphasic evidence from this study, it is proposed that the unknown bacterium should be classified in a new genus as a novel species, Ethanoligenens harbinense gen. nov., sp. nov. The type strain of Ethanoligenens harbinense is YUAN-3^T (=JCM 12961^T=CGMCC 1.5033^T).

Published online ahead of print on 25 November 2005 as DOI 10.1099/ijs.0.63926-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains YUAN-3^T and X-29 are AY295777 and AY833421, respectively.

Micrographs of colonies and cells of strain YUAN-3^T are available as supplementary material in IJSEM Online.

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