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Int J Syst Evol Microbiol 56 (2006), 721-725; DOI  10.1099/ijs.0.63686-0
© 2006 International Union of Microbiological Societies

Sporacetigenium mesophilum gen. nov., sp. nov., isolated from an anaerobic digester treating municipal solid waste and sewage

Shuangya Chen1,2, Lei Song1,2 and Xiuzhu Dong1

1 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China
2 Graduate School of Chinese Academy of Sciences, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China

Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn

Two mesophilic, anaerobic bacterial strains (ZLJ115T and L4-2) were isolated from the sludge of an anaerobic digester treating municipal solid waste and sewage in Fujian province, China. The strains were Gram-positive, spore-forming, motile rods (0·9–1·0x3·6–7·3 µm). Growth of the strains was observed at 20–42 °C and pH 6·0–9·5. Both strains fermented several mono- and disaccharides. The main fermentation products from glucose were acetate, ethanol, hydrogen and carbon dioxide. Optimal hydrogen production by the new isolates was observed at pH 8·8 and 39 °C, and 1·4 mol H2 was detected from fermentation of 1 mol glucose. The DNA G+C contents of strains ZLJ115T and L4-2 were 53·9 and 54·3 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represented a novel phyletic sublineage within cluster XI of the clostridia, clustering with four thermophilic species, with <93·8 % 16S rRNA gene sequence similarity to previously described species. Phenotypically, the new isolates were distinguished from their phylogenetic relatives by growing mesophilically and by fermenting a variety of pentoses, as well as their higher genome DNA G+C content. On the basis of polyphasic evidence from this study, a novel genus and species are proposed, Sporacetigenium mesophilum gen. nov., sp. nov.; strain ZLJ115T (=DSM 16796T=AS 1.5019T) is the type strain of Sporacetigenium mesophilum.

Abbreviations: DAP, diaminopimelic acid

Published online ahead of print on 2 December 2005 as DOI 10.1099/ijs.0.63686-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Sporacetigenium mesophilum ZLJ115T is AY682207.

Fatty acid profiles of the novel strains are available as supplementary material in IJSEM Online.






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