HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary Table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, S. Articles by Dong, X. Search for Related Content PubMed PubMed Citation Articles by Chen, S. Articles by Dong, X. Agricola Articles by Chen, S. Articles by Dong, X.

Sporacetigenium mesophilum gen. nov., sp. nov., isolated from an anaerobic digester treating municipal solid waste and sewage

¹ State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China
² Graduate School of Chinese Academy of Sciences, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, PR China

Correspondence
Xiuzhu Dong
dongxz{at}sun.im.ac.cn

Two mesophilic, anaerobic bacterial strains (ZLJ115^T and L4-2) were isolated from the sludge of an anaerobic digester treating municipal solid waste and sewage in Fujian province, China. The strains were Gram-positive, spore-forming, motile rods (0·9–1·0x3·6–7·3 µm). Growth of the strains was observed at 20–42 °C and pH 6·0–9·5. Both strains fermented several mono- and disaccharides. The main fermentation products from glucose were acetate, ethanol, hydrogen and carbon dioxide. Optimal hydrogen production by the new isolates was observed at pH 8·8 and 39 °C, and 1·4 mol H₂ was detected from fermentation of 1 mol glucose. The DNA G+C contents of strains ZLJ115^T and L4-2 were 53·9 and 54·3 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates represented a novel phyletic sublineage within cluster XI of the clostridia, clustering with four thermophilic species, with <93·8 % 16S rRNA gene sequence similarity to previously described species. Phenotypically, the new isolates were distinguished from their phylogenetic relatives by growing mesophilically and by fermenting a variety of pentoses, as well as their higher genome DNA G+C content. On the basis of polyphasic evidence from this study, a novel genus and species are proposed, Sporacetigenium mesophilum gen. nov., sp. nov.; strain ZLJ115^T (=DSM 16796^T=AS 1.5019^T) is the type strain of Sporacetigenium mesophilum.

Published online ahead of print on 2 December 2005 as DOI 10.1099/ijs.0.63686-0.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Sporacetigenium mesophilum ZLJ115^T is AY682207.

Fatty acid profiles of the novel strains are available as supplementary material in IJSEM Online.

This article has been cited by other articles:

				E. V. Pikuta, R. B. Hoover, D. Marsic, W. B. Whitman, B. Lupa, J. Tang, and P. Krader Proteocatella sphenisci gen. nov., sp. nov., a psychrotolerant, spore-forming anaerobe isolated from penguin guano Int J Syst Evol Microbiol, September 1, 2009; 59(9): 2302 - 2307. [Abstract] [Full Text] [PDF]