IJSEM Visit JGV Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figures and Tables
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Juvonen, R.
Right arrow Articles by Suihko, M.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Juvonen, R.
Right arrow Articles by Suihko, M.-L.
Agricola
Right arrow Articles by Juvonen, R.
Right arrow Articles by Suihko, M.-L.
Int J Syst Evol Microbiol 56 (2006), 695-702; DOI  10.1099/ijs.0.63699-0
© 2006 International Union of Microbiological Societies

Megasphaera paucivorans sp. nov., Megasphaera sueciensis sp. nov. and Pectinatus haikarae sp. nov., isolated from brewery samples, and emended description of the genus Pectinatus

Riikka Juvonen and Maija-Liisa Suihko

VTT Biotechnology, PO Box 1500, Espoo, FI-02044 VTT, Finland

Correspondence
Riikka Juvonen
riikka.juvonen{at}vtt.fi

Seven unidentified strictly anaerobic, Gram-negative, non-spore-forming bacteria from spoiled beer or the brewery environment were characterized. Based on 16S rRNA gene sequence analyses, all strains were affiliated to the Sporomusa sub-branch of the class ‘Clostridia’. Three of the strains were non-motile cocci, on average 1·5x1·2 µm or 1·2x1·0 µm, occurring mainly singly or in pairs. They shared nearly identical (>99 %) 16S rRNA gene sequences, being most closely related to the species of the Megasphaera–Anaeroglobus group (<=93·9 % similarity). According to DNA–DNA hybridization results, the coccoid strains represented two genospecies, neither of which was related to any of the recognized Megasphaera species. Several phenotypic characteristics and/or DNA G+C content also differentiated the strains from each other and from their closest relatives. The other four novel strains were motile, slightly curved to helical rods, 0·6–0·8x3–50 µm or more in size. They shared identical 16S rRNA gene sequences and ribofragment patterns. The highest 16S rRNA gene similarity was found between these isolates and Pectinatus cerevisiiphilus ATCC 29359T (95·6 %) and Pectinatus frisingensis ATCC 33332T (93·6 %). The novel strains also differed from recognized Pectinatus species in their sugar utilization, proteolytic activity, catalase activity, antibiotic resistance and temperature tolerance. The results suggest that the bacteria belong to three novel species, for which the names Megasphaera paucivorans sp. nov. (type strain VTT E-032341T=DSM 16981T), Megasphaera sueciensis sp. nov. (type strain VTT E-97791T=DSM 17042T) and Pectinatus haikarae sp. nov. (type strain VTT E-88329T=DSM 16980T) are proposed.

Abbreviations: ML, maximum-likelihood; MP, maximum-parsimony; NJ, neighbour-joining

Published online ahead of print on 9 December 2005 as DOI 10.1099/ijs.0.63699-0.

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains VTT E-97791T, VTT E-032341T, VTT E-88329T and VTT E-90406T are DQ223729, DQ223730, DQ223731 and DQ217599, respectively.

Results of ribotyping analysis and volatile fatty acid profiles of the novel strains are available as supplementary material in IJSEM Online.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2006 by the International Union of Microbiological Societies.